- Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
- Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition"Zeiss LSM 780 NLO
- Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
- Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.
Suitable for imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS.
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Directions
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3
Details
microscope
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Zeiss - Axio Examiner.Z1, Upright stand, motorized XY stage, motorized Z-drive,
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objectives
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- Zeiss EC Plan Neofluar 2.5x 0.075
- Zeiss Plan-Apochromat 10x 0.45
- Zeiss Plan-Apochromat 20x 0.8
- Zeiss Plan-Apochromat 40x 0,95 korr.
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- Zeiss W Plan-Apochromat 20x/1.0
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illumination
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- Fluorescence (Metal Halide, HXP, 120W)
- Transmitted Light (Halogen)
- Laser Diode 405 nm
- Laser Argon Multiline 458, 488, 514 nm
- Laser DPSS 561 nm
- Laser HeNe 633 nm
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- Laser Multiphoton Chameleon VisionII 690-1064nm
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detection
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Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting
2 photon detection: Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP and RFP
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reflectors
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Zeiss set 49: DAPI
Zeiss set 38: GFP
Zeiss set 43: Cy3
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features
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simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
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software
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Zen "black" 2011
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incubation
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Not available
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links
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- [ beam path]
- [ advanced system check]
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inv.nr.
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AIM System 2504000174
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