SD1 - Andor Spinning Disc
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Olympus - IX71, Inverted stand, manual, 1.6x OptoVar, fast piezo objective z-positioner (Physik Instrumente - PI), Prior ProScanIII xy scanning stage | ||
objectives | |||
illumination | |||
detection | [[detection::Spinning disc scan head Yokogawa CSU-X1 (10.000rpm): dichromatic mirrors: Quad band T-405/488/568/647 Triple band T-405/488/561 Single band T-488 physical pinhole radius: 25um, physical pinhole spacing: 250um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar) Andor Dual Camera Port TuCam with two cameras and 0.95x projecting lens for each camera: Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um | ||
reflectors | Optical Insight DualView image splitter (currently not installed): holder for GFP/mCherry: BL 525/40; BS 565; ET 605/70 holder for GFP/RFP: HC 520/35; BS 565; HC 628/40 Sutter filter wheel: position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: edge basic 635LP microscope filter turret: position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: cube with z375 RDC for micropoint cutter, 5: empty, 6: DIC analyzer | ||
features | micro point with 15Hz cutter laser with 365nm and 405nm dye cells;
objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!"micro point with 15Hz cutter laser with 365nm and 405nm dye cells; objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!" cannot be used as a page name in this wiki. | ||
software | [[software::iQ 2.9 + iQ 3.0 (both with Python) iQ3 user guide]] | ||
incubation | Stage incubator (T) + objective heater | ||
links |
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inv.nr. | 0 10.1.65.208 |