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| − | {{ASC | + | {{Asc |
| | | system-number = MPI09 | | | system-number = MPI09 |
| − | | description = Our [[Zeiss LSM 510]] [[confocal]] laser scanning microscope is designed for routine work and dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. | + | | running-time = 4:00 |
| − | | applications = -
| + | | temperature = 21 |
| − | | microscope = inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | + | | conditions = ok |
| − | | objectives = 10x/0.3<br>20x/0.5<br>40x/0.95 corr<br>40x/1.2 W corr<br>100x/1.4 Oil DIC | + | | comments = 405 illumination looks weird, might be spectral detector, needs to be confirmed |
| − | | illumination = HBO lamp<br>Halogen lamp<br>405 diode laser<br>458, 477(optional), 488, 514 argon laser<br>543 HeNe laser<br>633 HeNe laser | + | | illumination-violet = 2008-11-24_MPI09_illum_405.jpg |
| − | | detection = point scanner, 2 confocal PMTs, 1 META detector, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | + | | illumination-blue = 2008-11-24_MPI09_illum_488.jpg |
| − | | reflectors = DAPI : EX 365/12 ; BS 395 ; EM LP 397<br>FITC : EX 470/40 ; BS 510 ; EM LP 515<br>TRITC : EX 546/12 ; BS 580 ; EM LP 590 LP | + | | illumination-green = - |
| − | | features = simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP
| + | | illumination-red = - |
| − | | incubation = none
| + | |
| − | | link1 = [http://spreadsheets.google.com/pub?key=pMZJXCgd5HNvd5kdKaSffSQ LPM]
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| − | | link2 = - | + | |
| − | | inv = - | + | |
| | }} | | }} |
Latest revision as of 17:42, 25 November 2008
| system
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MPI09
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| running time
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4:00
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| temperature
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21
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| conditions
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ok
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| comments
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405 illumination looks weird, might be spectral detector, needs to be confirmed
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illumination of field
10x lens, open pinhole, mirror slide, reflection mode, maximum scan-field, 8 bit
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150px
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150px
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| ex 350-420nm
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ex 420-520nm
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ex 520-620nm
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ex 620-720nm
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|
uv/vis overlay
-
psf
-
