ASC Field Illumination
From BioDIP
(Difference between revisions)
(Created page with 'The two images acquired in the first step already gave an impression of the field illumination. Homogeneous illumination is particularly important for quantitative analysis, i.e....') |
|||
| Line 11: | Line 11: | ||
file:Field illumination zeiss mirror.jpg|Field Illumination: Zeiss mirror slide | file:Field illumination zeiss mirror.jpg|Field Illumination: Zeiss mirror slide | ||
</gallery> | </gallery> | ||
| + | __NOTOC__ | ||
Revision as of 14:19, 30 September 2009
The two images acquired in the first step already gave an impression of the field illumination. Homogeneous illumination is particularly important for quantitative analysis, i.e. the comparison of intensities in different regions of the field of view. Therefore the following test provides a more quantitative analysis of the field illumination.
requirements
- mirror slide
- alternatively: reflective surface (i.e. glass slide, gold-coated surface)
acquisition
- 80/20 mirror, 488 nm laser, pinhole 1 AU
- focus on reflective side of mirror
- try to obtain "japanese flag"
images
Field Illumination: Zeiss mirror slide
