Advanced system check
From BioDIP
(Difference between revisions)
(New page: MPI-CBG LMF Advanced System Check Protocol (draft) *fluorescence and bright field imaging (all systems) **purpose: test general performance of the system - basic functions work fine? **...) |
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**purpose: test general performance of the system - basic functions work fine? | **purpose: test general performance of the system - basic functions work fine? | ||
**requirements: Zeiss/Leica Convallaria demo sample | **requirements: Zeiss/Leica Convallaria demo sample | ||
| − | **work flow: acquire two images in basic resolution ( | + | **work flow: acquire two images in basic resolution (512x512), blue ex / green em plus transmitted light image, using 10x objective |
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*psf | *psf | ||
**purpose: | **purpose: | ||
Revision as of 14:05, 21 September 2009
MPI-CBG LMF
Advanced System Check
Protocol (draft)
- fluorescence and bright field imaging (all systems)
- purpose: test general performance of the system - basic functions work fine?
- requirements: Zeiss/Leica Convallaria demo sample
- work flow: acquire two images in basic resolution (512x512), blue ex / green em plus transmitted light image, using 10x objective
- psf
- purpose:
- requirements:
- work flow:
- overlay UV/V and VIS (confocal systems with separate UV/V laser coupling)
- purpose: check the coupling precision of the UV/V laser
- requirements: 0.2/0.5µm fluorescent beads sample
- work flow: open pinhole(s), use high resolution apochromatic lens (NA 1.2 or above), acquire two images (UV/V plus VIS channel) with good sampling (pixel size ~100nm)
