Advanced system check
From BioDIP
(Difference between revisions)
| Line 6: | Line 6: | ||
*fluorescence and bright field imaging (all systems) | *fluorescence and bright field imaging (all systems) | ||
| − | **purpose: test general performance of the system - basic functions work fine? | + | **purpose: test general performance of the system - basic functions work fine? + Koehler illumination, light paths ok? |
**requirements: Zeiss/Leica Convallaria demo sample | **requirements: Zeiss/Leica Convallaria demo sample | ||
**work flow: acquire two images in basic resolution (512x512), blue ex / green em plus transmitted light image, using 10x objective | **work flow: acquire two images in basic resolution (512x512), blue ex / green em plus transmitted light image, using 10x objective | ||
Revision as of 14:06, 21 September 2009
MPI-CBG LMF
Advanced System Check
Protocol (draft)
- fluorescence and bright field imaging (all systems)
- purpose: test general performance of the system - basic functions work fine? + Koehler illumination, light paths ok?
- requirements: Zeiss/Leica Convallaria demo sample
- work flow: acquire two images in basic resolution (512x512), blue ex / green em plus transmitted light image, using 10x objective
- psf
- purpose:
- requirements:
- work flow:
- overlay UV/V and VIS (confocal systems with separate UV/V laser coupling)
- purpose: check the coupling precision of the UV/V laser
- requirements: 0.2/0.5µm fluorescent beads sample
- work flow: open pinhole(s), use high resolution apochromatic lens (NA 1.2 or above), acquire two images (UV/V plus VIS channel) with good sampling (pixel size ~100nm)
