ApoTome1, inverse, BIOTEC
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|system-name=ApoTome1, inverse, BIOTEC | |system-name=ApoTome1, inverse, BIOTEC | ||
|type=Microscope | |type=Microscope | ||
− | |category= | + | |category=Structured Illumination |
− | |facility=Imaging@ | + | |facility=Imaging@BIOTEC/CRTD |
− | |building= | + | |building=BIOTEC |
|room=225 | |room=225 | ||
|Facility-logo=Imaging@Biotec.jpg | |Facility-logo=Imaging@Biotec.jpg | ||
− | |Institute-logo= | + | |Institute-logo=Biotec logo.png |
− | |description=Our | + | |description=Our Apotome microscope inverted is designed for routine work on fixed sample. Optical sectioning can be obtained on fixed samples by the use of the ApoTome. |
|applications=- | |applications=- | ||
− | |image= | + | |image=ApoTome1, inverse, BIOTEC.jpg |
− | |stand-name=Zeiss - | + | |stand-name=Zeiss - Axiovert 200M |
|stand=Inverted | |stand=Inverted | ||
− | |microscope= | + | |microscope=automatic XY stage, motorized z-stage, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (Apotome), Mosaix (tile scans) |
− | |obj1=Zeiss | + | |obj1=Zeiss EC Plan-Neofluar 10x 0.3 Ph1 |
− | |obj2=Zeiss | + | |obj2=Zeiss Plan-Apochromat 20x 0.8 |
− | |obj3=Zeiss | + | |obj3=Zeiss Plan-Apochromat 40x 0,95 korr. |
|obj4=Zeiss EC Plan-Neofluar 40x 1.3 Oil | |obj4=Zeiss EC Plan-Neofluar 40x 1.3 Oil | ||
− | |obj5=Zeiss Plan-Apochromat 63x 1.4 Oil | + | |obj5=Zeiss Plan-Apochromat 63x 1.4 Oil Ph3 |
− | |obj6=Zeiss Plan- | + | |obj6=Zeiss alpha Plan-Fluar 100x 1.45 Oil |
+ | |obj7=Zeiss EC Plan Neofluar 5x 0.16 | ||
+ | |obj8=Zeiss EC Plan Neofluar 2.5x 0.075 | ||
|ill1=Fluorescence (Metal Halide, HXP, 120W) | |ill1=Fluorescence (Metal Halide, HXP, 120W) | ||
|ill2=Transmitted Light (Halogen) | |ill2=Transmitted Light (Halogen) | ||
− | |detection=Axiocam | + | |detection=Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w<br> |
− | |reflectors=DAPI : EX 360/40 ; BS 400 ; EM 460/50<br> | + | Axiocam MRc (1388*1040, 6,45*6,45µm pixel) - color |
+ | |reflectors=DAPI : EX 360/40 ; BS 400 ; EM 460/50<br> | ||
+ | EGFP : EX 470/40 ; BS 495 ; EM 525/50<br> | ||
+ | Cy3 : EX 545/25 ; BS 565 ; EM 605/70<br> | ||
+ | Cy5 : EX 620/60 ; BS 660 ; EM 700/75 | ||
|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, micromanipulator | |features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, micromanipulator | ||
− | |software= | + | |software=32 bit: AxioVision |
+ | 64 bit: Win7 ZEN blue 2012 | ||
|incubation=Not available | |incubation=Not available | ||
|link1=- | |link1=- | ||
|link2=- | |link2=- | ||
− | |inv= | + | |inv=121643 |
|facility_image=logo-imagingatbiotec.jpg | |facility_image=logo-imagingatbiotec.jpg | ||
|objectives=10x/0.3 Ph1<br>10x/0.45 W<br>20x/0.4 Ph2 Corr LD<br>40x/1.3 Oil DIC<br>63x/1.4 Oil DIC | |objectives=10x/0.3 Ph1<br>10x/0.45 W<br>20x/0.4 Ph2 Corr LD<br>40x/1.3 Oil DIC<br>63x/1.4 Oil DIC | ||
|illumination=X-Cite Series 120<br>Halogen | |illumination=X-Cite Series 120<br>Halogen | ||
}} | }} |
Latest revision as of 10:46, 13 February 2017
[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
microscope | Zeiss - Axiovert 200M, Inverted stand, automatic XY stage, motorized z-stage, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (Apotome), Mosaix (tile scans) | ||
objectives | |||
illumination | |||
detection | Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w Axiocam MRc (1388*1040, 6,45*6,45µm pixel) - color | ||
reflectors | DAPI : EX 360/40 ; BS 400 ; EM 460/50 EGFP : EX 470/40 ; BS 495 ; EM 525/50 | ||
features | sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, micromanipulator | ||
software | 32 bit: AxioVision
64 bit: Win7 ZEN blue 2012"32 bit: AxioVision 64 bit: Win7 ZEN blue 2012" cannot be used as a page name in this wiki. | ||
incubation | Not available | ||
links |
| ||
inv.nr. | 121643 |