ApoTome1, inverse, BIOTEC

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|stand-name=Zeiss - Axiovert 200M
 
|stand-name=Zeiss - Axiovert 200M
 
|stand=Inverted
 
|stand=Inverted
|microscope=automatic XY stage, motorized z-stage,, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (Apotome), Mosaix (tile scans)
+
|microscope=automatic XY stage, motorized z-stage, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (Apotome), Mosaix (tile scans)
|obj1=Zeiss C-Apochromat 10x 0.45 W
+
|obj1=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
|obj2=Zeiss LD Achroplan 20x 0.4 Ph2
+
|obj2=Zeiss Plan-Apochromat 20x 0.8
|obj3=Zeiss LD Achroplan 40x 0.6 Ph2
+
|obj3=Zeiss Plan-Apochromat 40x 0,95 korr.
 
|obj4=Zeiss EC Plan-Neofluar 40x 1.3 Oil
 
|obj4=Zeiss EC Plan-Neofluar 40x 1.3 Oil
|obj5=Zeiss Plan-Apochromat 63x 1.4 Oil
+
|obj5=Zeiss Plan-Apochromat 63x 1.4 Oil Ph3
|obj6=Zeiss Plan-Apochromat 100x 1.4 Oil
+
|obj6=Zeiss alpha Plan-Fluar 100x 1.45 Oil
|obj7=Zeiss Plan-Apochromat 40x 0,95 korr.
+
|obj7=Zeiss EC Plan Neofluar 5x 0.16
 +
|obj8=Zeiss EC Plan Neofluar 2.5x 0.075
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill2=Transmitted Light (Halogen)
 
|ill2=Transmitted Light (Halogen)
|detection=Axiocam HR (1300*1030, 6,7*6,7µm pixel) - b/w<br>
+
|detection=Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w<br>
 
Axiocam MRc (1388*1040, 6,45*6,45µm pixel) - color
 
Axiocam MRc (1388*1040, 6,45*6,45µm pixel) - color
 
|reflectors=DAPI : EX 360/40 ; BS 400 ; EM 460/50<br>
 
|reflectors=DAPI : EX 360/40 ; BS 400 ; EM 460/50<br>
GFP : EX 470/40 ; BS 495 ; EM 525/50<br>
+
EGFP : EX 470/40 ; BS 495 ; EM 525/50<br>
 
Cy3 : EX 545/25 ; BS 565 ; EM 605/70<br>
 
Cy3 : EX 545/25 ; BS 565 ; EM 605/70<br>
 
Cy5 : EX 620/60 ; BS 660 ; EM 700/75
 
Cy5 : EX 620/60 ; BS 660 ; EM 700/75
 
|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, micromanipulator
 
|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, micromanipulator
|software=Axiovision
+
|software=32 bit: AxioVision
 +
64 bit: Win7 ZEN blue 2012
 
|incubation=Not available
 
|incubation=Not available
 
|link1=-
 
|link1=-

Latest revision as of 10:46, 13 February 2017

Suitable for -.
ApoTome1, inverse, BIOTEC.jpg


[edit] Directions

Biotec logo.png
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (BIOTEC, 225)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

[edit] Details

microscope Zeiss - Axiovert 200M, Inverted stand, automatic XY stage, motorized z-stage, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (Apotome), Mosaix (tile scans)
objectives
Zeiss EC Plan-Neofluar 10x 0.3 Ph1
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss EC Plan-Neofluar 40x 1.3 Oil
Zeiss Plan-Apochromat 63x 1.4 Oil Ph3
Zeiss alpha Plan-Fluar 100x 1.45 Oil
Zeiss EC Plan Neofluar 5x 0.16
Zeiss EC Plan Neofluar 2.5x 0.075
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w

Axiocam MRc (1388*1040, 6,45*6,45µm pixel) - color

reflectors DAPI : EX 360/40 ; BS 400 ; EM 460/50

EGFP : EX 470/40 ; BS 495 ; EM 525/50
Cy3 : EX 545/25 ; BS 565 ; EM 605/70
Cy5 : EX 620/60 ; BS 660 ; EM 700/75

features sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, micromanipulator
software 32 bit: AxioVision

64 bit: Win7 ZEN blue 2012"32 bit: AxioVision 64 bit: Win7 ZEN blue 2012" cannot be used as a page name in this wiki.

incubation Not available
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 121643



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