ApoTome1, inverse, BIOTEC

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{{Equipment
 
{{Equipment
| system-number       = 13
+
|system-number=BIO03
| system-name         = Videomicroscope Microinjection Apotome
+
|system-name=ApoTome1, inverse, BIOTEC
| category            = Wide-Field
+
|type=Microscope
| stand                = inverted
+
|category=Structured Illumination
| image                = Bioz13.jpg
+
|facility=Imaging@BIOTEC/CRTD
| facility             = Imaging@Biotec
+
|building=BIOTEC
| facility_image      = logo-imagingatbiotec.jpg
+
|room=225
| building            = Biotec
+
|Facility-logo=Imaging@Biotec.jpg
| room                = 225
+
|Institute-logo=Biotec logo.png
| description         = Our microinjection Apotome videomicroscope inverted is designed for routine work on fixed sample and micromanipulation. Optical sectioning can be obtained on fixed samples by the use of the Apotome.
+
|description=Our Apotome microscope inverted is designed for routine work on fixed sample. Optical sectioning can be obtained on fixed samples by the use of the ApoTome.
| microscope          = inverted stand, manual XY stage, motorized z-stage, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (Apotome)
+
|applications=-
| objectives          = 10x/0.3 Ph1<br>20x/0.4 Ph2 Corr LD<br>40x/1.3 Oil DIC<br>63x/1.4 Oil DIC
+
|image=ApoTome1, inverse, BIOTEC.jpg
| illumination        = X-Cite Series 120<br>Halogen
+
|stand-name=Zeiss - Axiovert 200M
| detection           = Axiocam HR (1300*1030, 6,7*6,7µm pixel)<br>Apotome
+
|stand=Inverted
| reflectors           = DAPI : EX 360/40 ; BS 400 ; EM 460/50<br>GFP : EX 470/40 ; BS 495 ; EM 525/50<br>Cy3 : EX 545/25 ; BS 565 ; EM 605/70<br>Cy5 : EX 620/60 ; BS 660 ; EM 700/75
+
|microscope=automatic XY stage, motorized z-stage, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (Apotome), Mosaix (tile scans)
| features             = sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, micromanipulator
+
|obj1=Zeiss EC Plan-Neofluar 10x 0.3 Ph1
| incubation           = None
+
|obj2=Zeiss Plan-Apochromat 20x 0.8
 +
|obj3=Zeiss Plan-Apochromat 40x 0,95 korr.
 +
|obj4=Zeiss EC Plan-Neofluar 40x 1.3 Oil
 +
|obj5=Zeiss Plan-Apochromat 63x 1.4 Oil Ph3
 +
|obj6=Zeiss alpha Plan-Fluar 100x 1.45 Oil
 +
|obj7=Zeiss EC Plan Neofluar 5x 0.16
 +
|obj8=Zeiss EC Plan Neofluar 2.5x 0.075
 +
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 +
|ill2=Transmitted Light (Halogen)
 +
|detection=Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w<br>
 +
Axiocam MRc (1388*1040, 6,45*6,45µm pixel) - color
 +
|reflectors=DAPI : EX 360/40 ; BS 400 ; EM 460/50<br>
 +
EGFP : EX 470/40 ; BS 495 ; EM 525/50<br>
 +
Cy3 : EX 545/25 ; BS 565 ; EM 605/70<br>
 +
Cy5 : EX 620/60 ; BS 660 ; EM 700/75
 +
|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, micromanipulator
 +
|software=32 bit: AxioVision
 +
64 bit: Win7 ZEN blue 2012
 +
|incubation=Not available
 +
|link1=-
 +
|link2=-
 +
|inv=121643
 +
|facility_image=logo-imagingatbiotec.jpg
 +
|objectives=10x/0.3 Ph1<br>10x/0.45 W<br>20x/0.4 Ph2 Corr LD<br>40x/1.3 Oil DIC<br>63x/1.4 Oil DIC
 +
|illumination=X-Cite Series 120<br>Halogen
 
}}
 
}}

Latest revision as of 10:46, 13 February 2017

Suitable for -.
ApoTome1, inverse, BIOTEC.jpg


[edit] Directions

Biotec logo.png
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (BIOTEC, 225)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

[edit] Details

microscope Zeiss - Axiovert 200M, Inverted stand, automatic XY stage, motorized z-stage, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (Apotome), Mosaix (tile scans)
objectives
Zeiss EC Plan-Neofluar 10x 0.3 Ph1
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss EC Plan-Neofluar 40x 1.3 Oil
Zeiss Plan-Apochromat 63x 1.4 Oil Ph3
Zeiss alpha Plan-Fluar 100x 1.45 Oil
Zeiss EC Plan Neofluar 5x 0.16
Zeiss EC Plan Neofluar 2.5x 0.075
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w

Axiocam MRc (1388*1040, 6,45*6,45µm pixel) - color

reflectors DAPI : EX 360/40 ; BS 400 ; EM 460/50

EGFP : EX 470/40 ; BS 495 ; EM 525/50
Cy3 : EX 545/25 ; BS 565 ; EM 605/70
Cy5 : EX 620/60 ; BS 660 ; EM 700/75

features sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, micromanipulator
software 32 bit: AxioVision

64 bit: Win7 ZEN blue 2012"32 bit: AxioVision 64 bit: Win7 ZEN blue 2012" cannot be used as a page name in this wiki.

incubation Not available
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 121643



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