ApoTome1, inverse, BIOTEC

From BioDIP
(Difference between revisions)
Jump to: navigation, search
Line 24: Line 24:
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill2=Transmitted Light (Halogen)
 
|ill2=Transmitted Light (Halogen)
|detection=Axiocam HR (1300*1030, 6,7*6,7µm pixel) - b/w<br>
+
|detection=Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w<br>
 
Axiocam MRc (1388*1040, 6,45*6,45µm pixel) - color
 
Axiocam MRc (1388*1040, 6,45*6,45µm pixel) - color
 
|reflectors=DAPI : EX 360/40 ; BS 400 ; EM 460/50<br>
 
|reflectors=DAPI : EX 360/40 ; BS 400 ; EM 460/50<br>

Revision as of 12:55, 6 December 2013

Suitable for -.
ApoTome1, inverse, BIOTEC.jpg


Directions

Biotec logo.png
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (BIOTEC, 225)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Zeiss - Axiovert 200M, Inverted stand, automatic XY stage, motorized z-stage,, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (Apotome), Mosaix (tile scans)
objectives
Zeiss C-Apochromat 10x 0.45 W
Zeiss LD Achroplan 20x 0.4 Ph2
Zeiss LD Achroplan 40x 0.6 Ph2
Zeiss EC Plan-Neofluar 40x 1.3 Oil
Zeiss Plan-Apochromat 63x 1.4 Oil
Zeiss Plan-Apochromat 100x 1.4 Oil
Zeiss Plan-Apochromat 40x 0,95 korr.
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w

Axiocam MRc (1388*1040, 6,45*6,45µm pixel) - color

reflectors DAPI : EX 360/40 ; BS 400 ; EM 460/50

GFP : EX 470/40 ; BS 495 ; EM 525/50
Cy3 : EX 545/25 ; BS 565 ; EM 605/70
Cy5 : EX 620/60 ; BS 660 ; EM 700/75

features sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, micromanipulator
software Axiovision
incubation Not available
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 121643



Personal tools