ApoTome1, inverse, BIOTEC
From BioDIP
(Difference between revisions)
| Line 15: | Line 15: | ||
|stand=Inverted | |stand=Inverted | ||
|microscope=automatic XY stage, motorized z-stage,, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (Apotome), Mosaix (tile scans) | |microscope=automatic XY stage, motorized z-stage,, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (Apotome), Mosaix (tile scans) | ||
| − | |obj1=Zeiss | + | |obj1=Zeiss EC Plan-Neofluar 10x 0.3 Ph1 |
| − | |obj2=Zeiss | + | |obj2=Zeiss Plan-Apochromat 20x 0.8 |
| − | |obj3=Zeiss | + | |obj3=Zeiss Plan-Apochromat 40x 0,95 korr. |
|obj4=Zeiss EC Plan-Neofluar 40x 1.3 Oil | |obj4=Zeiss EC Plan-Neofluar 40x 1.3 Oil | ||
|obj5=Zeiss Plan-Apochromat 63x 1.4 Oil | |obj5=Zeiss Plan-Apochromat 63x 1.4 Oil | ||
| − | |obj6=Zeiss Plan- | + | |obj6=Zeiss alpha Plan-Fluar 100x 1.45 Oil |
| − | + | ||
|ill1=Fluorescence (Metal Halide, HXP, 120W) | |ill1=Fluorescence (Metal Halide, HXP, 120W) | ||
|ill2=Transmitted Light (Halogen) | |ill2=Transmitted Light (Halogen) | ||
| Line 27: | Line 26: | ||
Axiocam MRc (1388*1040, 6,45*6,45µm pixel) - color | Axiocam MRc (1388*1040, 6,45*6,45µm pixel) - color | ||
|reflectors=DAPI : EX 360/40 ; BS 400 ; EM 460/50<br> | |reflectors=DAPI : EX 360/40 ; BS 400 ; EM 460/50<br> | ||
| − | + | EGFP : EX 470/40 ; BS 495 ; EM 525/50<br> | |
Cy3 : EX 545/25 ; BS 565 ; EM 605/70<br> | Cy3 : EX 545/25 ; BS 565 ; EM 605/70<br> | ||
Cy5 : EX 620/60 ; BS 660 ; EM 700/75 | Cy5 : EX 620/60 ; BS 660 ; EM 700/75 | ||
|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, micromanipulator | |features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, micromanipulator | ||
| − | |software= | + | |software=32 bit: AxioVision |
| + | 64 bit: Win7 ZEN blue 2012 | ||
|incubation=Not available | |incubation=Not available | ||
|link1=- | |link1=- | ||
Revision as of 17:34, 20 May 2014
|
Directions
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
Details
| microscope | Zeiss - Axiovert 200M, Inverted stand, automatic XY stage, motorized z-stage,, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (Apotome), Mosaix (tile scans) | ||
| objectives | |||
| illumination | |||
| detection | Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w Axiocam MRc (1388*1040, 6,45*6,45µm pixel) - color | ||
| reflectors | DAPI : EX 360/40 ; BS 400 ; EM 460/50 EGFP : EX 470/40 ; BS 495 ; EM 525/50 | ||
| features | sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, micromanipulator | ||
| software | 32 bit: AxioVision
64 bit: Win7 ZEN blue 2012"32 bit: AxioVision 64 bit: Win7 ZEN blue 2012" cannot be used as a page name in this wiki. | ||
| incubation | Not available | ||
| links |
| ||
| inv.nr. | 121643 |
