ApoTome1 2nd floor, inv., CRTD

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(Created page with '{{Equipment |system-number=BIO01 |system-name=ApoTome1 2nd floor, inv., CRTD |type=Microscope |category=Wide-Field |facility=Imaging@Biotec |building=CRTD |room=2.230 |Facility-l...')
 
 
(14 intermediate revisions by 3 users not shown)
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|system-name=ApoTome1 2nd floor, inv., CRTD
 
|system-name=ApoTome1 2nd floor, inv., CRTD
 
|type=Microscope
 
|type=Microscope
|category=Wide-Field
+
|category=Structured Illumination
|facility=Imaging@Biotec
+
|facility=Imaging@BIOTEC/CRTD
 
|building=CRTD
 
|building=CRTD
 
|room=2.230
 
|room=2.230
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|description=It is designed for routine work on fixed sample. Optical sectioning can be obtained on fixed samples by the use of the ApoTome1.
 
|description=It is designed for routine work on fixed sample. Optical sectioning can be obtained on fixed samples by the use of the ApoTome1.
 
|applications=-
 
|applications=-
|stand-name=Zeiss - Axio Observer
+
|image=ApoTome1 2nd floor, inv., CRTD.jpg
 +
|stand-name=Zeiss - Axio Observer.Z1
 
|stand=Inverted
 
|stand=Inverted
 
|microscope=motorized XY stage, motorized z-stage, fluorescence, transmitted light with DIC, b/w epifluorescence acquisition, Optical Sectioning (ApoTome1)
 
|microscope=motorized XY stage, motorized z-stage, fluorescence, transmitted light with DIC, b/w epifluorescence acquisition, Optical Sectioning (ApoTome1)
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|obj3=Zeiss EC Plan-Neofluar 40x 1.3 Oil
 
|obj3=Zeiss EC Plan-Neofluar 40x 1.3 Oil
 
|obj4=Zeiss Plan-Apochromat 63x 1.4 Oil
 
|obj4=Zeiss Plan-Apochromat 63x 1.4 Oil
 +
|obj5=Zeiss Plan-Apochromat 40x 0,95 korr.
 +
|obj6=Zeiss EC Plan Neofluar 2.5x 0.075
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill2=Transmitted Light (Halogen)
 
|ill2=Transmitted Light (Halogen)
 
|detection=Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w
 
|detection=Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w
|reflectors=FS 49: EX BP G365; BS 395; EM BP 445/50 (Dapi)<br>
+
|reflectors=FS 49: EX BP 347/55; BS LP 395; EM BP 445/50 (Dapi)<br>
FS 38 HE: EX BP 470/40; BS LP 495; BP 525/50 (GFP) <br>
+
FS 38 HE: EX BP 470/40; BS LP 495; EM BP 525/50 (GFP) <br>
FS 43 HE: EX BP 550/25; BS LP 570; BP 605/70 (Cy3) <br>
+
FS 43 HE: EX BP 550/25; BS LP 570; EM BP 605/70 (Cy3) <br>
FS 50 HE: EX BP 640/30; BS LP 660; BP 690/50 (Cy5) <br>
+
FS 50 HE: EX BP 640/30; BS LP 660; EM BP 690/50 (Cy5) <br>
Yellow<br>
+
FS 46 ET: EX BP 436/20; BS LP 455; EM BP 480/40 (CFP) <br>
FS Analysator for DIC and Transmission  
+
FS Analysator for DIC and Transmission
 
+
|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, mosaix scan
|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, mosaix scan  
+
|software=32 bit: AxioVision
|software=Axiovision
+
64 bit: Win7 ZEN blue 2012
 
|incubation=Not available
 
|incubation=Not available
 
|inv=67051/00
 
|inv=67051/00
 
}}
 
}}

Latest revision as of 00:15, 8 February 2015

Suitable for -.
ApoTome1 2nd floor, inv., CRTD.jpg


[edit] Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, 2.230)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

[edit] Details

microscope Zeiss - Axio Observer.Z1, Inverted stand, motorized XY stage, motorized z-stage, fluorescence, transmitted light with DIC, b/w epifluorescence acquisition, Optical Sectioning (ApoTome1)
objectives
Zeiss EC Plan-Neofluar 10x 0.3
Zeiss Plan-Apochromat 20x 0.8
Zeiss EC Plan-Neofluar 40x 1.3 Oil
Zeiss Plan-Apochromat 63x 1.4 Oil
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss EC Plan Neofluar 2.5x 0.075
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w
reflectors FS 49: EX BP 347/55; BS LP 395; EM BP 445/50 (Dapi)

FS 38 HE: EX BP 470/40; BS LP 495; EM BP 525/50 (GFP)
FS 43 HE: EX BP 550/25; BS LP 570; EM BP 605/70 (Cy3)
FS 50 HE: EX BP 640/30; BS LP 660; EM BP 690/50 (Cy5)
FS 46 ET: EX BP 436/20; BS LP 455; EM BP 480/40 (CFP)
FS Analysator for DIC and Transmission

features sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, mosaix scan
software 32 bit: AxioVision

64 bit: Win7 ZEN blue 2012"32 bit: AxioVision 64 bit: Win7 ZEN blue 2012" cannot be used as a page name in this wiki.

incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 67051/00



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