ApoTome1 3rd floor, upr., CRTD

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|detection=Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w<br>
 
|detection=Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w<br>
 
Axiocam MRc (1388*1040 pixel, 6,45*6,45µm) - color
 
Axiocam MRc (1388*1040 pixel, 6,45*6,45µm) - color
−
|reflectors=FS 49: EX BP G365; BS 395; EM BP 445/50 (Dapi)<br>
+
|reflectors=F46-000 Dapi-ET: EX AT 350/50; LP 400; EM BP 460/50<br>
−
FS HC-FITC: EX BP 482/35 (HE); BS 505 (HE); EM BP 536/40 (HE); (GFP)<br>
+
EGFP-ET: EX BP 470/40; LP 495; EM BP 525/36<br>
−
FS 43HE: EX BP 550/25 (HE); BS 570 (HE); EM 605/70 (HE); (Cy3)<br>
+
F46-004 Cy3-ET: EX BP 545/25; LP 565; EM BP 605/70<br>
−
FS Cy5 HC: EX BP 640/30 ; BS 660 ; EM 690/50; (Cy5)<br>
+
F46-006 Cy5-ET : EX BP 620/60; LP 660; EM BP 700/75<br>
−
FS 24: EX BP 485/20 - BP 578/14; BS 500 - LP 600; EM 527/25 - LP 610; (GFP-RFP)<br>
+
F46-003 YFP-ET: EX BP 500/20; LP 515; EM BP 535/30<br>
 
FS Analysator for DIC and Transmission
 
FS Analysator for DIC and Transmission
 
|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, mosaix scan
 
|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, mosaix scan

Revision as of 11:53, 8 May 2014

Suitable for -.
ApoTome1 3rd floor, upr., CRTD.jpg


Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, 3.235)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Zeiss - Axio Imager, Upright stand, motorized XY stage, motorized z-stage, fluorescence, transmitted light with DIC, b/w epifluorescence acquisition, Optical Sectioning (Apotome), color acquisition for stained histological samples
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss EC Plan-Neofluar 40x 1.3 Oil
Zeiss EC Plan Neofluar 2.5x 0.075
Zeiss Achroplan 40x 0.8 W
Zeiss Plan-Apochromat 5x/0.16
Zeiss C-Apochromat 63x 1.2 W
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w

Axiocam MRc (1388*1040 pixel, 6,45*6,45µm) - color

reflectors F46-000 Dapi-ET: EX AT 350/50; LP 400; EM BP 460/50

EGFP-ET: EX BP 470/40; LP 495; EM BP 525/36
F46-004 Cy3-ET: EX BP 545/25; LP 565; EM BP 605/70
F46-006 Cy5-ET : EX BP 620/60; LP 660; EM BP 700/75
F46-003 YFP-ET: EX BP 500/20; LP 515; EM BP 535/30
FS Analysator for DIC and Transmission

features sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, mosaix scan
software 32 bit: AxioVision

64 bit: Win7 ZEN blue 2012"32 bit: AxioVision 64 bit: Win7 ZEN blue 2012" cannot be used as a page name in this wiki.

incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 163533 (old)


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