ApoTome1 3rd floor, upr., CRTD

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(Created page with '{{Equipment |system-number=BIO16 |system-name=Apotome @ first floor |type=Microscope |category=Wide-Field |facility=Imaging@Biotec |building=Biotec |room=115 |Facility-logo=Imagi...')
 
 
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{{Equipment
 
{{Equipment
|system-number=BIO16
+
|system-number=BIO02
|system-name=Apotome @ first floor
+
|system-name=ApoTome1 3rd floor, upr., CRTD
 
|type=Microscope
 
|type=Microscope
|category=Wide-Field
+
|category=Structured Illumination
|facility=Imaging@Biotec
+
|facility=Imaging@BIOTEC/CRTD
|building=Biotec
+
|building=CRTD
|room=115
+
|room=3.235
 
|Facility-logo=Imaging@Biotec.jpg
 
|Facility-logo=Imaging@Biotec.jpg
 
|Institute-logo=CRTD logo.PNG
 
|Institute-logo=CRTD logo.PNG
 
|description=It is designed for routine work on fixed sample. Optical sectioning can be obtained on fixed samples by the use of the Apotome.
 
|description=It is designed for routine work on fixed sample. Optical sectioning can be obtained on fixed samples by the use of the Apotome.
 
|applications=-
 
|applications=-
 +
|image=ApoTome1 3rd floor, upr., CRTD.jpg
 
|stand-name=Zeiss - Axio Imager
 
|stand-name=Zeiss - Axio Imager
 
|stand=Upright
 
|stand=Upright
 
|microscope=motorized XY stage, motorized z-stage, fluorescence, transmitted light with DIC, b/w epifluorescence acquisition, Optical Sectioning (Apotome), color acquisition for stained histological samples
 
|microscope=motorized XY stage, motorized z-stage, fluorescence, transmitted light with DIC, b/w epifluorescence acquisition, Optical Sectioning (Apotome), color acquisition for stained histological samples
 +
|obj1=Zeiss EC Plan Neofluar 2.5x 0.075
 +
|obj2=Zeiss Plan-Apochromat 5x/0.16
 
|obj3=Zeiss Plan-Apochromat 10x 0.45
 
|obj3=Zeiss Plan-Apochromat 10x 0.45
 
|obj4=Zeiss Plan-Apochromat 20x 0.8
 
|obj4=Zeiss Plan-Apochromat 20x 0.8
|obj5=Zeiss Plan-Apochromat 40x 0,95 korr.
+
|obj5=Zeiss Achroplan 40x 0.75 W
|obj6=Zeiss EC Plan-Neofluar 40x 1.3 Oil
+
|obj6=Zeiss Plan-Apochromat 40x 0,95 korr.
 +
|obj7=Zeiss C-Apochromat 63x 1.2 W
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill2=Transmitted Light (Halogen)
 
|ill2=Transmitted Light (Halogen)
|detection=Axiocam MR (1388*1040, 6,45*6,45µm pixel) - b/w<br>
+
|detection=Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w<br>
Axiocam MRc (1388*1040, 6,45*6,45µm pixel) - color
+
Axiocam MRc (1388*1040 pixel, 6,45*6,45µm) - color
|reflectors=FS 49: EX BP G365; BS 395; EM BP 445/50 (Dapi)<br>
+
|reflectors=F46-000 Dapi-ET: EX AT 350/50; BS LP 400; EM BP 460/50<br>
FS 38HE: EX BP 470/40 (HE); BS 495 (HE); EM BP 525/50 (HE); (GFP)<br>
+
EGFP-ET: EX BP 470/40; BS LP 495; EM BP 525/36<br>
FS 43HE: EX BP 550/25 (HE); BS 570 (HE); EM 605/70 (HE); (Cy3)<br>
+
F46-004 Cy3-ET: EX BP 545/25; BS LP 565; EM BP 605/70<br>
FS 50: EX BP 640/30 ; BS 660 ; EM 690/50; (Cy5)
+
F46-006 Cy5-ET : EX BP 620/60; BS LP 660; EM BP 700/75<br>
 +
F46-003 YFP-ET: EX BP 500/20; BS LP 515; EM BP 535/30<br>
 +
Analysator for DIC and Transmission
 
|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, mosaix scan
 
|features=sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, mosaix scan
|software=Axiovision
+
|software=32 bit: AxioVision
 +
64 bit: Win7 ZEN blue 2012
 
|incubation=Not available
 
|incubation=Not available
|inv=0
+
|inv=163533 (old)
 
}}
 
}}

Latest revision as of 13:29, 17 October 2014

Suitable for -.
ApoTome1 3rd floor, upr., CRTD.jpg


[edit] Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, 3.235)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

[edit] Details

microscope Zeiss - Axio Imager, Upright stand, motorized XY stage, motorized z-stage, fluorescence, transmitted light with DIC, b/w epifluorescence acquisition, Optical Sectioning (Apotome), color acquisition for stained histological samples
objectives
Zeiss EC Plan Neofluar 2.5x 0.075
Zeiss Plan-Apochromat 5x/0.16
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Achroplan 40x 0.75 W
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss C-Apochromat 63x 1.2 W
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection Axiocam MRm (1388*1040, 6,45*6,45µm) - b/w

Axiocam MRc (1388*1040 pixel, 6,45*6,45µm) - color

reflectors F46-000 Dapi-ET: EX AT 350/50; BS LP 400; EM BP 460/50

EGFP-ET: EX BP 470/40; BS LP 495; EM BP 525/36
F46-004 Cy3-ET: EX BP 545/25; BS LP 565; EM BP 605/70
F46-006 Cy5-ET : EX BP 620/60; BS LP 660; EM BP 700/75
F46-003 YFP-ET: EX BP 500/20; BS LP 515; EM BP 535/30
Analysator for DIC and Transmission

features sequential imaging, Brightfield and Fluorescence overlay, timelapse, z-stack, optical sectioning, mosaix scan
software 32 bit: AxioVision

64 bit: Win7 ZEN blue 2012"32 bit: AxioVision 64 bit: Win7 ZEN blue 2012" cannot be used as a page name in this wiki.

incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 163533 (old)



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