BasicsCoursePhDprog-PeterEvennetMay2011-observed timing

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Revision as of 14:39, 17 May 2011

Contents

PhD Program: Basics of Light Microscopy - Peter Evennett / LMF - Course May 2011

Setup -Fri 13 May

Setup - all lmf staff move stuff to galleria Equipment needed

  • Microscopes
  • Optical bench
  • Spinning disk gadget
  • Polarazing sheets
  • Diffraction Grids
  • Fluorescent samples
  • Fluorescent Beads (Prepare slides)
  • First day Handout for students
  • Light torches


DAY ONE (16th May)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 09:30 FINAL PREP Test everything works LMF TEAM
9:30 10:15 INTRODUCTION COURSE INTRO
WHO is WHO? Round table
LMF TEAM
10:15 10:55 Session 1
Theory
PRINCIPLES OF LIGHT MICROSCOPY
Historical aspects
Very basic components and principles ( incl. Refraction-basics 1st part)
PETER EVENNETT
10:55 11:15 BREAK BREAK BREAK
11:20 12:45 SESSION 1
Theory
Refraction-basics 2nd part (Coin-in-cup-demo)
Introduction to lenses (Lens demo fun)
Lens aberrations (see colour fringes of lighting image with simple lens)
Magnification
Resolution, Numerical aperture
PETER EVENNETT
12:45 13:45 LUNCH LUNCH LUNCH
13:45 14:45 SESSION 1
Practice
15min (!!!) Rotation
Milky Glass Block demo
Show and discuss microscope parts (upright and inverted)
NA measurements
DAN
JAN & BRITTA
SILKE
14:45 15:30 SESSION 2
Theory
Microscope illumination
Conjugate planes
PETER EVENNETT
15:30 15:45 BREAK BREAK BREAK
15:45 16:50 SESSION 2
Practice
(30min Rotation)
conjugate planes on Peters microscope
conjugate planes on optical bench
PETER EVENNETT
JAN
16:50 17:15 SESSION 3
Theory
Koehler Illumination
(Microscope alignment)
PETER EVENNETT
17:15 18:30 SESSION 3
Practice
Koehler illumination
(Microscope alignment)
LMF TEAM

DAY TWO (May 17th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 10:00 Recap day 1 LMF TEAM
10:00 10:20 SESSION 1
Theory & Practice
Depth of Field
Depth of Focus
PETER EVENNETT
LMF TEAM
10:20 11:15 SESSION 2
Theory & Practice
Diffraction
Diffraction slide fun
PETER EVENNETT
11:15 11:30 BREAK COFFEE BREAK Note: have break earlier next time, right after diffraction-string-show
(should be shortly before 11am)
11:30 13:15 DEMONSTRATION ABBE'S DIFFRACTION EXPERIMENT
Peter's Video (50min)
Dan's setup demonstration
PETER EVENNETT
DAN
12:50 13:50 BREAK LUNCH
13:50 14:05 spontaneous discussion :-) PETER EVENNETT
LMF TEAM
14:05 14: SESSION 3
Theory
Lens aberrations and corrections
Objectives
PETER EVENNETT
SESSION 4
Theory
Bright Field
Dark Field
PETER EVENNETT
SESSION 4
Practice
Bright Field
Dark Field
LMF TEAM
16:15 16:30 BREAK COFFEE BREAK
16:30 17:15 SESSION 5
Theory
Phase Contrast microscopy PETER EVENNETT
17:15 18:15 SESSION 5
Practice
Phase Contrast microscopy LMF TEAM
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