BasicsCoursePhDprog-PeterEvennetMay2011-observed timing

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Revision as of 18:37, 17 May 2011

Contents

PhD Program: Basics of Light Microscopy - Peter Evennett / LMF - Course May 2011

Setup -Fri 13 May

Setup - all lmf staff move stuff to galleria Equipment needed

  • Microscopes
  • Optical bench
  • Spinning disk gadget
  • Polarazing sheets
  • Diffraction Grids
  • Fluorescent samples
  • Fluorescent Beads (Prepare slides)
  • First day Handout for students
  • Light torches


DAY ONE (16th May)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 09:30 FINAL PREP Test everything works LMF TEAM
9:30 10:15 INTRODUCTION COURSE INTRO
WHO is WHO? Round table
LMF TEAM
10:15 10:55 Session 1
Theory
PRINCIPLES OF LIGHT MICROSCOPY
Historical aspects
Very basic components and principles ( incl. Refraction-basics 1st part)
PETER EVENNETT
10:55 11:15 BREAK BREAK BREAK
11:20 12:45 SESSION 1
Theory
Refraction-basics 2nd part (Coin-in-cup-demo)
Introduction to lenses (Lens demo fun)
Lens aberrations (see colour fringes of lighting image with simple lens)
Magnification
Resolution, Numerical aperture
PETER EVENNETT
12:45 13:45 LUNCH LUNCH LUNCH
13:45 14:45 SESSION 1
Practice
15min (!!!) Rotation
Milky Glass Block demo
Show and discuss microscope parts (upright and inverted)
NA measurements
DAN
JAN & BRITTA
SILKE
14:45 15:30 SESSION 2
Theory
Microscope illumination
Conjugate planes
PETER EVENNETT
15:30 15:45 BREAK BREAK BREAK
15:45 16:50 SESSION 2
Practice
(30min Rotation)
conjugate planes on Peters microscope
conjugate planes on optical bench
PETER EVENNETT
JAN
16:50 17:15 SESSION 3
Theory
Koehler Illumination
(Microscope alignment)
PETER EVENNETT
17:15 18:30 SESSION 3
Practice
Koehler illumination
(Microscope alignment)
LMF TEAM

DAY TWO (May 17th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 10:00 Recap day 1 LMF TEAM
10:00 10:20 SESSION 1
Theory & Practice
Depth of Field
Depth of Focus
PETER EVENNETT
LMF TEAM
10:20 11:15 SESSION 2
Theory & Demo
Diffraction
Diffraction slide fun
PETER EVENNETT
11:15 11:30 BREAK COFFEE BREAK Note: have break earlier next time, right after diffraction-string-show
(should be shortly before 11am)
then do all the explanation of Abbe's Diffraction Exp after break
11:30 12:50 DEMONSTRATION ABBE'S DIFFRACTION EXPERIMENT
Peter's Video (50min)
Dan's setup demonstration
PETER EVENNETT
DAN
12:50 13:50 BREAK LUNCH
13:50 14:05 spontaneous discussion :-)
14:05 14:55 SESSION 3
Theory
Lens aberrations and corrections
Objectives
PETER EVENNETT
14:55 15:10 BREAK COFFEE BREAK
15:10 15:55 SESSION 4
Theory
Dark Field microscopy PETER EVENNETT
15:55 16:30 SESSION 4
Practice
Dark Field microscopy LMF TEAM
16:30 16:50 SESSION 5
Theory
Basics of Phase Contrast microscopy PETER EVENNETT
16:50 17:30 SESSION 5
Practice
Phase Contrast microscopy LMF TEAM
17:30  : SESSION 5
Theory
Details of Phase Contrast microscopy PETER EVENNETT


DAY THREE (May 18th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:45 Recap day 2 LMF TEAM
9:45 10:45 SESSION 1
Theory
Objective reading
Objective cleaning
PETER EVENNETT
LMF TEAM
10:45 11:00 BREAK BREAK BREAK
11:00 12:00 SESSION 1
Practice
Group rotation
Cover glasses and sample mounting
Objective reading
Objective cleaning
Dan
Britta, Silke
Jan
12:00 13:15 SESSION 2
Theory & Practice
Polarized light microscopy PETER EVENNETT
LMF TEAM
13:15 14:15 BREAK LUNCH
14:15 15:30 SESSION 3
Theory & Practice
Differential Interference Contrast (DIC) PETER EVENNETT
LMF TEAM
15:30 15:45 BREAK BREAK BREAK
15:45 16:15 Question round Questions to Peter Evennett PETER EVENNETT
LMF TEAM
16:15 18:15 SESSION 4
Theory & Demo
What is a pixel
Analog to Digital conversion
Nuyquist-Shannon
Dan
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