BasicsCoursePhDprog-PeterEvennetMay2011-observed timing

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| style="background:mediumseagreen;"|'''13:45'''
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|align="center" style="background:DarkKhaki;"|'''LUNCH'''
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|align="center" style="background:mediumseagreen;"|'''LUNCH'''
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|align="center" style="background:DarkKhaki;"|'''LUNCH'''||align="center" style="background:DarkKhaki;"|'''LUNCH'''
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|align="center" style="background:mediumseagreen;"|'''LUNCH'''||align="center" style="background:mediumseagreen;"|'''LUNCH'''
 
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| 13:45||14:45||align="center"|SESSION 1<br>Practice<br> 15min (!!!) Rotation|| align="center" |Milky Glass Block demo <br>Show and discuss microscope parts (upright and inverted)<br>NA measurements||align="center" |DAN<br>JAN & BRITTA<br>SILKE
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| style="background:LemonChiffon;"| 13:45|| style="background:LemonChiffon;"| 14:45
 +
|align="center" style="background:LemonChiffon;"|SESSION 1<br>Practice<br> 15min (!!!) Rotation|| align="center" style="background:LemonChiffon;"|Milky Glass Block demo <br>Show and discuss microscope parts (upright and inverted)<br>NA measurements||align="center" style="background:LemonChiffon;"|DAN<br>JAN & BRITTA<br>SILKE
 
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|14:45|| 15:30||align="center"|SESSION 2<br>Theory||align="center" |Microscope illumination <br> Conjugate planes||align="center" |PETER EVENNETT
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|14:45|| 15:30||align="center"|SESSION 2<br>Theory||align="center"|Microscope illumination <br> Conjugate planes||align="center" |PETER EVENNETT
 
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| style="background:DarkKhaki;"|'''15:30'''
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| style="background:mediumseagreen;"|'''15:30'''
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| style="background:DarkKhaki;"|'''15:45'''
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| style="background:mediumseagreen;"|'''15:45'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''||align="center" style="background:DarkKhaki;"|'''BREAK'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''||align="center" style="background:mediumseagreen;"|'''BREAK'''
 
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|15:45||16:50||align="center"|SESSION 2<br>Practice<br> (30min Rotation)|| align="center" |conjugate planes on Peters microscope <br> conjugate planes on optical bench||align="center" |PETER EVENNETT<br>JAN
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|style="background:LemonChiffon;"|15:45
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|style="background:LemonChiffon;"|16:50 || align="center" style="background:LemonChiffon;"|SESSION 2<br>Practice<br> (30min Rotation)|| align="center" style="background:LemonChiffon;"|conjugate planes on Peters microscope <br> conjugate planes on optical bench||align="center" style="background:LemonChiffon;"|PETER EVENNETT<br>JAN
 
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|17:15||18:30||align="center"|SESSION 3<br>Practice|| align="center" |Koehler illumination<br>(Microscope alignment)||align="center" |LMF TEAM
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|style="background:LemonChiffon;"|17:15
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|style="background:LemonChiffon;"|18:30||align="center" style="background:LemonChiffon;"|SESSION 3<br>Practice|| align="center" style="background:LemonChiffon;"|Koehler illumination<br>(Microscope alignment)||align="center" style="background:LemonChiffon;"|LMF TEAM
 
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| 10:00||10:20||align="center"|SESSION 1 <br>Theory & Practice||align="center" |  Depth of Field<br>Depth of Focus  ||align="center" |PETER EVENNETT <br>LMF TEAM
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|style="background:LemonChiffon;"| 10:00
 +
|style="background:LemonChiffon;"|10:20||align="center" style="background:LemonChiffon;"|SESSION 1 <br>Theory & Practice||align="center" style="background:LemonChiffon;"|  Depth of Field<br>Depth of Focus  ||align="center" style="background:LemonChiffon;"|PETER EVENNETT <br>LMF TEAM
 
|-
 
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| 10:20||11:15||align="center"|SESSION 2<br>Theory & Practice||align="center" | Diffraction <br> Diffraction slide fun  ||align="center" |PETER EVENNETT
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| 10:20||11:15||align="center"|SESSION 2<br>Theory & Demo||align="center" | Diffraction <br> Diffraction slide fun  ||align="center" |PETER EVENNETT
 
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|-
 
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|--
 
|--
 
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| style="background:DarkKhaki;"|'''11:15'''
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| style="background:mediumseagreen;"|'''11:15'''
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| style="background:DarkKhaki;"|'''11:30'''
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| style="background:mediumseagreen;"|'''11:30'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''
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|align="center" style="background:DarkKhaki;"|'''COFFEE BREAK'''|| align="center" style="background:DarkKhaki;"|''Note: have break earlier next time, right after diffraction-string-show <br> (should be shortly before 11am)''
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|align="center" style="background:mediumseagreen;"|'''COFFEE BREAK'''|| align="center" style="background:mediumseagreen;"|''Note: have break earlier next time, right after diffraction-string-show <br> (should be shortly before 11am)<br>then do all the explanation of Abbe's Diffraction Exp after break''
 
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| 11:30||13:15||align="center"|DEMONSTRATION || align="center"|ABBE'S DIFFRACTION EXPERIMENT<br>Peter's Video (50min) <br> Dan's setup demonstration||align="center"|PETER EVENNETT<br>DAN
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| 11:30||12:50||align="center"|DEMONSTRATION || align="center"|ABBE'S DIFFRACTION EXPERIMENT<br>Peter's Video (50min) <br> Dan's setup demonstration||align="center"|PETER EVENNETT<br>DAN
 
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| style="background:DarkKhaki;"|'''12:50'''
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| style="background:mediumseagreen;"|'''12:50'''
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| style="background:DarkKhaki;"|'''13:50'''
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| style="background:mediumseagreen;"|'''13:50'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''
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|align="center" style="background:DarkKhaki;"|'''LUNCH'''|| style="background:DarkKhaki;"|'''    '''
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|align="center" style="background:mediumseagreen;"|'''LUNCH'''|| style="background:mediumseagreen;"|'''    '''
 
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| 13:50||14:05||align="center"|  || align="center"|spontaneous discussion :-) ||align="center" |PETER EVENNETT<br>LMF TEAM
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| 13:50||14:05||align="center"|  || align="center"|spontaneous discussion :-) ||
 
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| style="background:DarkKhaki;"|'''14:55'''
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| style="background:mediumseagreen;"|'''14:55'''
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| style="background:DarkKhaki;"|'''15:10'''
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| style="background:mediumseagreen;"|'''15:10'''
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|align="center" style="background:DarkKhaki;"|'''BREAK'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''
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|align="center" style="background:DarkKhaki;"|'''COFFEE BREAK'''|| style="background:DarkKhaki;"|'''    '''
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|align="center" style="background:mediumseagreen;"|'''COFFEE BREAK'''|| style="background:mediumseagreen;"|'''    '''
 
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| 15:10 || ||align="center"|SESSION 4<br>Theory|| align="center"|Bright Field<br> Dark Field||align="center" |PETER EVENNETT
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| 15:10 || 15:55 ||align="center"|SESSION 4<br>Theory|| align="center"| Dark Field microscopy||align="center" |PETER EVENNETT
 
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|   || ||align="center"|SESSION 4<br>Practice|| align="center"|Bright Field<br> Dark Field||align="center" |LMF TEAM
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|style="background:LemonChiffon;"| 15:55
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|style="background:LemonChiffon;"| 16:30 ||align="center" style="background:LemonChiffon;"|SESSION 4<br>Practice|| align="center" style="background:LemonChiffon;"|Dark Field microscopy||align="center" style="background:LemonChiffon;"|LMF TEAM
 
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| style="background:DarkKhaki;"|'''16:15'''
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| 16:30||16:50||align="center"|SESSION 5<br>Theory|| align="center"|Basics of Phase Contrast microscopy ||align="center" |PETER EVENNETT
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| style="background:DarkKhaki;"|'''16:30'''
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−
|align="center" style="background:DarkKhaki;"|'''BREAK'''
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|align="center" style="background:DarkKhaki;"|'''COFFEE BREAK'''|| style="background:DarkKhaki;"|'''    '''
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|-
 
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| 16:30||17:15||align="center"|SESSION 5<br>Theory|| align="center"|Phase Contrast microscopy ||align="center" |PETER EVENNETT
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|style="background:LemonChiffon;"| 16:50
 +
|style="background:LemonChiffon;"|17:30||align="center" style="background:LemonChiffon;"|SESSION 5<br>Practice|| align="center" style="background:LemonChiffon;"|Phase Contrast microscopy ||align="center" style="background:LemonChiffon;"|LMF TEAM
 
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| 17:15||18:15||align="center"|SESSION 5<br>Practice|| align="center"|Phase Contrast microscopy ||align="center" |LMF TEAM
+
| 17:30|| : ||align="center"|SESSION 5<br>Theory|| align="center"|Details of Phase Contrast microscopy ||align="center" |PETER EVENNETT
 +
|}
 +
 
 +
 
 +
=== DAY THREE (May 18th)===
 +
 
 +
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
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|- style="; color:MidnightBlue;"
 +
| align="center" style="background:#f0f0f0;"|'''FROM'''
 +
| align="center" style="background:#f0f0f0;"|'''TO'''
 +
| align="center" style="background:#f0f0f0;"|'''ACTIVITY'''
 +
| align="center" style="background:#f0f0f0;"|'''TOPICS'''
 +
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 +
|-
 +
| 9:00||10:00||align="center"|Recap ||align="center" |  day 2  ||align="center" |LMF TEAM
 +
|-
 +
| 10:00||10:20||align="center"|SESSION 1<br>Theory||align="center" | Objective reading||align="center" |PETER EVENNETT <br>LMF TEAM
 +
|-
 +
|-
 +
| style="background:mediumseagreen;"|'''10:20'''
 +
| style="background:mediumseagreen;"|'''10:35'''
 +
|align="center" style="background:mediumseagreen;"|'''BREAK'''
 +
|align="center" style="background:mediumseagreen;"|'''BREAK'''|| align="center" style="background:mediumseagreen;"|'''BREAK'''
 +
|-
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|-
 +
|style="background:LemonChiffon;"| 10:40
 +
|style="background:LemonChiffon;"|12:10||align="center" style="background:LemonChiffon;"|SESSION 1<br>Practice<br>Group rotation (30min. each!)||align="center" style="background:LemonChiffon;"| Cover glasses and sample mounting<br>Objective reading<br> Objective cleaning||align="center" style="background:LemonChiffon;"|Dan<br>Britta, Silke<br>Jan
 +
|-
 +
|-
 +
| 12:10||13:00||align="center"|SESSION 2<br>Theory||align="center" | Polarized light microscopy||align="center" |PETER EVENNETT
 +
|-
 +
|-
 +
| style="background:mediumseagreen;"|'''13:00'''
 +
| style="background:mediumseagreen;"|'''14:00'''
 +
|align="center" style="background:mediumseagreen;"|'''BREAK'''
 +
|align="center" style="background:mediumseagreen;"|'''LUNCH'''|| style="background:mediumseagreen;"|'''    '''
 +
|-
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|-
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|style="background:LemonChiffon;"| 14:00
 +
|style="background:LemonChiffon;"|14:40||align="center" style="background:LemonChiffon;"|SESSION 2<br>Practice||align="center" style="background:LemonChiffon;"| Polarized light microscopy||align="center"  style="background:LemonChiffon;"|LMF TEAM
 +
|-
 +
|-
 +
| 14:40||15:05||align="center"|SESSION 3<br>Theory|| align="center"|Differential Interference Contrast (DIC)||align="center" |PETER EVENNETT
 +
|-
 +
|-
 +
|style="background:LemonChiffon;"| 15:05
 +
|style="background:LemonChiffon;"|15:40||align="center" style="background:LemonChiffon;"|SESSION 3<br>Practice|| align="center" style="background:LemonChiffon;"|Differential Interference Contrast (DIC)||align="center" style="background:LemonChiffon;"|LMF TEAM
 +
|-
 +
|-
 +
| style="background:mediumseagreen;"|'''15:40'''
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| style="background:mediumseagreen;"|'''16:00'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''|| align="center" style="background:mediumseagreen;"|'''BREAK'''
 +
|-
 +
| 16:00||16:30||align="center"|Question round|| align="center"|Questions to Peter Evennett||align="center" |PETER EVENNETT <br>LMF TEAM
 +
|-
 +
| 16:30||17:40||align="center"|SESSION 4<br>Theory & Demo|| align="center"|What is a pixel<br>Analog to Digital conversion<br>Nuyquist-Shannon||align="center" |Dan
 +
|-
 +
|-
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|style="background:LemonChiffon;"| 17:40
 +
|style="background:LemonChiffon;"|18:00||align="center" style="background:LemonChiffon;"|SESSION 3<br>Practice|| align="center" style="background:LemonChiffon;"|PIXELS<br> Sesame seeds, sunflower seeds, nudels, gumibears and a checker board||align="center" style="background:LemonChiffon;"|LMF TEAM
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|-
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|}
 +
 
 +
=== DAY FOUR (May 19th)===
 +
 
 +
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
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|- style="; color:MidnightBlue;"
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| align="center" style="background:#f0f0f0;"|'''FROM'''
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| align="center" style="background:#f0f0f0;"|'''TO'''
 +
| align="center" style="background:#f0f0f0;"|'''ACTIVITY'''
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| align="center" style="background:#f0f0f0;"|'''TOPICS'''
 +
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 +
| align="center" style="background:#f0f0f0;"|'''DEMO Activities'''
 +
| align="center" style="background:#f0f0f0;"|'''DEMO materials'''
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|-
 +
| 9:00||10:00||align="center"|Recap ||align="center" |  day 3  ||align="center" |LMF TEAM ||||
 +
|-
 +
| 10:00||10:45||align="center"|SESSION 1<br>Theory||align="center" | Fundamental concepts of fluorescence ||align="center" |DAN||align="center" |- ||align="center" |-
 +
|-
 +
|-
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| style="background:mediumseagreen;"|'''10:45'''
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| style="background:mediumseagreen;"|'''11:00'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''|| align="center" style="background:mediumseagreen;"|'''BREAK'''||align="center" style="background:mediumseagreen;"|'''BREAK'''||align="center" style="background:mediumseagreen;"|'''BREAK'''
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|-
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|-
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| 11:00||12:15||align="center"|SESSION 2<br>Theory & Practice||align="center" | Fluorescence Microscopy||align="center" |SILKE<br>LMFTEAM||align="center" |• Light sources<br>• Lamp houses <br>• Spectra <br>• Filters <br> • Filter cubes <br> • Filter spectral charts of each microscope (laminated)||align="center" | • Spare lamp houses <br> • Lamps <br> • Ocean optics equipment <br> • Four (4) different Filter-sets (cubes), with the appropriate descriptions <br> • diagramms for drawing filter/fluorophore spectra
 +
|-
 +
|-
 +
| 12:15||13:00||align="center"|SESSION 2<br>Practice||align="center" | Fluorescence imaging at microscopes ||align="center" |LMF TEAM||align="center" |- ||align="center" |• Fluorescent labeled samples
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|-
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|-
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| style="background:mediumseagreen;"|'''13:00'''
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| style="background:mediumseagreen;"|'''14:00'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''
 +
|align="center" style="background:mediumseagreen;"|'''LUNCH'''|| align="center" style="background:mediumseagreen;"|'''LUNCH'''||align="center" style="background:mediumseagreen;"|'''LUNCH'''||align="center" style="background:mediumseagreen;"|'''LUNCH'''
 +
|-
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|-
 +
| 14:00||15:45||align="center"|SESSION 3<br>Theory & Practice|| align="center"|Imaging with CCD and Quantitative Imaging ||align="center" |LMF TEAM<br> Britta/Dan||align="center" | • CCD<br> • Cameras || align="center" |• CCD chip <br> • RGB Slider Spot Camera setup
 +
|-
 +
| style="background:mediumseagreen;"|'''15:45'''
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| style="background:mediumseagreen;"|'''16:00'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''|| style="background:mediumseagreen;"|'''    '''|| style="background:mediumseagreen;"|'''    '''|| style="background:mediumseagreen;"|'''    '''
 +
|-
 +
| 16:00||18:00||align="center"|SESSION 4<br>Practice|| align="center"|Imaging with CCD cameras using LMF systems ||align="center" |LMF TEAM ||align="center"|• Displaying <br>• Pixel size<br>• Look up table<br>• Binning<br>• Exposure time<br>• Saturation<br>• Signal to noise <br>• ROI<br>• Bit depth<br>• Auto map<br>• Auto contrast || align="center"|• Microscopes: <br> DVcore(Dan, 3 students)<br> pDV (Silke, 2 students)<br> Inverted CCD (Pete, 2 students)<br> N-Storm (Britta, 2 students) <br>self-built-syst (Jan, 3 students)) <br>• Fluorescence Sample slides (Kidney / Cells)
 +
|-
 +
|-
 +
| style="background:SkyBlue;"|'''19:00'''
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| style="background:SkyBlue;"|'''open end'''
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|align="center" style="background:SkyBlue;"|'''Dinner'''
 +
|align="center" style="background:SkyBlue;"|'''with Peter'''|| align="center" style="background:SkyBlue;"|'''LMF TEAM'''|| style="background:SkyBlue;"|'''    '''|| style="background:SkyBlue;"|'''    '''
 +
|}
 +
''
 +
 
 +
=== DAY FIVE (May 20th)===
 +
 
 +
{| border="4"  cellpadding="2" cellspacing="8" {| {{table}}
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|- style="; color:MidnightBlue;"
 +
| align="center" style="background:#f0f0f0;"|'''FROM'''
 +
| align="center" style="background:#f0f0f0;"|'''TO'''
 +
| align="center" style="background:#f0f0f0;"|'''ACTIVITY'''
 +
| align="center" style="background:#f0f0f0;"|'''TOPICS'''
 +
| align="center" style="background:#f0f0f0;"|'''Responsible person(s)'''
 +
|-
 +
| 9:00||10:00||align="center"|Recap ||align="center" |  day 4  ||align="center" |LMF TEAM
 +
|-
 +
|10:00||11:10||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan
 +
|-
 +
|-
 +
| style="background:mediumseagreen;"|'''11:10'''
 +
| style="background:mediumseagreen;"|'''11:25'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''
 +
|align="center" style="background:mediumseagreen;"|'''BREAK'''||align="center" style="background:mediumseagreen;"|'''BREAK'''
 +
|-
 +
|-
 +
| 11:25||13:00||align="center"|SESSION 1<br>Theory & Demo||align="center" | Optical sectioning methods<br>Pros&Cons ||align="center" |LMF TEAM <br> Dan / Jan
 +
|-
 +
|-
 +
| style="background:mediumseagreen;"|'''13:00'''
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| style="background:mediumseagreen;"|'''14:00'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''
 +
|align="center" style="background:mediumseagreen;"|'''LUNCH'''|| style="background:mediumseagreen;"|'''    '''
 +
|-
 +
|-
 +
| 14:00||15:00||align="center"|SESSION 2<br>Small Groups Discussion|| align="center"|What techniques might be good for me<br> (Own Projects) ||align="center" |LMF TEAM<br>Dan/Jan/Silke/Britta/Pete?
 +
|-
 +
|-
 +
| style="background:mediumseagreen;"|'''15:00'''
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| style="background:mediumseagreen;"|'''15:15'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''
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|align="center" style="background:mediumseagreen;"|'''BREAK'''|| align="center" style="background:mediumseagreen;"|'''BREAK'''
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|-
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|-
 +
| 15:15||16:00||align="center"|SESSION 3|| align="center"|Course evaluation ||align="center" |LMF TEAM
 
|}
 
|}

Latest revision as of 12:47, 20 May 2011

Contents

[edit] PhD Program: Basics of Light Microscopy - Peter Evennett / LMF - Course May 2011

[edit] Setup -Fri 13 May

Setup - all lmf staff move stuff to galleria Equipment needed

  • Microscopes
  • Optical bench
  • Spinning disk gadget
  • Polarazing sheets
  • Diffraction Grids
  • Fluorescent samples
  • Fluorescent Beads (Prepare slides)
  • First day Handout for students
  • Light torches


[edit] DAY ONE (16th May)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 09:30 FINAL PREP Test everything works LMF TEAM
9:30 10:15 INTRODUCTION COURSE INTRO
WHO is WHO? Round table		 LMF TEAM
10:15 10:55 Session 1
Theory		 PRINCIPLES OF LIGHT MICROSCOPY
Historical aspects
Very basic components and principles ( incl. Refraction-basics 1st part)		 PETER EVENNETT
10:55 11:15 BREAK BREAK BREAK
11:20 12:45 SESSION 1
Theory		 Refraction-basics 2nd part (Coin-in-cup-demo)
Introduction to lenses (Lens demo fun)
Lens aberrations (see colour fringes of lighting image with simple lens)
Magnification
Resolution, Numerical aperture		 PETER EVENNETT
12:45 13:45 LUNCH LUNCH LUNCH
13:45 14:45 SESSION 1
Practice
15min (!!!) Rotation		 Milky Glass Block demo
Show and discuss microscope parts (upright and inverted)
NA measurements		 DAN
JAN & BRITTA
SILKE
14:45 15:30 SESSION 2
Theory		 Microscope illumination
Conjugate planes		 PETER EVENNETT
15:30 15:45 BREAK BREAK BREAK
15:45 16:50 SESSION 2
Practice
(30min Rotation)		 conjugate planes on Peters microscope
conjugate planes on optical bench		 PETER EVENNETT
JAN
16:50 17:15 SESSION 3
Theory		 Koehler Illumination
(Microscope alignment)		 PETER EVENNETT
17:15 18:30 SESSION 3
Practice		 Koehler illumination
(Microscope alignment)		 LMF TEAM

[edit] DAY TWO (May 17th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 10:00 Recap day 1 LMF TEAM
10:00 10:20 SESSION 1
Theory & Practice		 Depth of Field
Depth of Focus 		PETER EVENNETT
LMF TEAM
10:20 11:15 SESSION 2
Theory & Demo		 Diffraction
Diffraction slide fun 		PETER EVENNETT
11:15 11:30 BREAK COFFEE BREAK Note: have break earlier next time, right after diffraction-string-show
(should be shortly before 11am)
then do all the explanation of Abbe's Diffraction Exp after break
11:30 12:50 DEMONSTRATION ABBE'S DIFFRACTION EXPERIMENT
Peter's Video (50min)
Dan's setup demonstration		 PETER EVENNETT
DAN
12:50 13:50 BREAK LUNCH
13:50 14:05 spontaneous discussion :-)
14:05 14:55 SESSION 3
Theory 		Lens aberrations and corrections
Objectives 		PETER EVENNETT
14:55 15:10 BREAK COFFEE BREAK
15:10 15:55 SESSION 4
Theory		 Dark Field microscopy PETER EVENNETT
15:55 16:30 SESSION 4
Practice		 Dark Field microscopy LMF TEAM
16:30 16:50 SESSION 5
Theory		 Basics of Phase Contrast microscopy PETER EVENNETT
16:50 17:30 SESSION 5
Practice		 Phase Contrast microscopy LMF TEAM
17:30  : SESSION 5
Theory		 Details of Phase Contrast microscopy PETER EVENNETT


[edit] DAY THREE (May 18th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 10:00 Recap day 2 LMF TEAM
10:00 10:20 SESSION 1
Theory		 Objective reading PETER EVENNETT
LMF TEAM
10:20 10:35 BREAK BREAK BREAK
10:40 12:10 SESSION 1
Practice
Group rotation (30min. each!)		 Cover glasses and sample mounting
Objective reading
Objective cleaning		 Dan
Britta, Silke
Jan
12:10 13:00 SESSION 2
Theory		 Polarized light microscopy PETER EVENNETT
13:00 14:00 BREAK LUNCH
14:00 14:40 SESSION 2
Practice		 Polarized light microscopy LMF TEAM
14:40 15:05 SESSION 3
Theory		 Differential Interference Contrast (DIC) PETER EVENNETT
15:05 15:40 SESSION 3
Practice		 Differential Interference Contrast (DIC) LMF TEAM
15:40 16:00 BREAK BREAK BREAK
16:00 16:30 Question round Questions to Peter Evennett PETER EVENNETT
LMF TEAM
16:30 17:40 SESSION 4
Theory & Demo		 What is a pixel
Analog to Digital conversion
Nuyquist-Shannon		 Dan
17:40 18:00 SESSION 3
Practice		 PIXELS
Sesame seeds, sunflower seeds, nudels, gumibears and a checker board		 LMF TEAM

[edit] DAY FOUR (May 19th)

FROM TO ACTIVITY TOPICS Responsible person(s) DEMO Activities DEMO materials
9:00 10:00 Recap day 3 LMF TEAM
10:00 10:45 SESSION 1
Theory		 Fundamental concepts of fluorescence DAN - -
10:45 11:00 BREAK BREAK BREAK BREAK BREAK
11:00 12:15 SESSION 2
Theory & Practice		 Fluorescence Microscopy SILKE
LMFTEAM		 • Light sources
• Lamp houses
• Spectra
• Filters
• Filter cubes
• Filter spectral charts of each microscope (laminated)		 • Spare lamp houses
• Lamps
• Ocean optics equipment
• Four (4) different Filter-sets (cubes), with the appropriate descriptions
• diagramms for drawing filter/fluorophore spectra
12:15 13:00 SESSION 2
Practice		 Fluorescence imaging at microscopes LMF TEAM - • Fluorescent labeled samples
13:00 14:00 BREAK LUNCH LUNCH LUNCH LUNCH
14:00 15:45 SESSION 3
Theory & Practice		 Imaging with CCD and Quantitative Imaging LMF TEAM
Britta/Dan		 • CCD
• Cameras 		• CCD chip
• RGB Slider Spot Camera setup
15:45 16:00 BREAK BREAK
16:00 18:00 SESSION 4
Practice		 Imaging with CCD cameras using LMF systems LMF TEAM • Displaying
• Pixel size
• Look up table
• Binning
• Exposure time
• Saturation
• Signal to noise
• ROI
• Bit depth
• Auto map
• Auto contrast 		• Microscopes:
DVcore(Dan, 3 students)
pDV (Silke, 2 students)
Inverted CCD (Pete, 2 students)
N-Storm (Britta, 2 students)
self-built-syst (Jan, 3 students))
• Fluorescence Sample slides (Kidney / Cells)
19:00 open end Dinner with Peter LMF TEAM

[edit] DAY FIVE (May 20th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 10:00 Recap day 4 LMF TEAM
10:00 11:10 SESSION 1
Theory & Demo		 Optical sectioning methods
Pros&Cons 		LMF TEAM
Dan / Jan
11:10 11:25 BREAK BREAK BREAK
11:25 13:00 SESSION 1
Theory & Demo		 Optical sectioning methods
Pros&Cons 		LMF TEAM
Dan / Jan
13:00 14:00 BREAK LUNCH
14:00 15:00 SESSION 2
Small Groups Discussion		 What techniques might be good for me
(Own Projects) 		LMF TEAM
Dan/Jan/Silke/Britta/Pete?
15:00 15:15 BREAK BREAK BREAK
15:15 16:00 SESSION 3 Course evaluation LMF TEAM
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