BasicsCoursePhDprog-PeterEvennetOct2011-observed timing and needed materials
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| align="center" style="background:#f0f0f0;"|'''Materials needed''' | | align="center" style="background:#f0f0f0;"|'''Materials needed''' | ||
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| − | | 9:00||10:00||align="center"|Recap ||align="center" | day 1 ||align="center" |LMF TEAM | + | | 9:00||10:00||align="center"|Recap ||align="center" | day 1 ||align="center" |LMF TEAM || |
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|style="background:LemonChiffon;"| 10:00 | |style="background:LemonChiffon;"| 10:00 | ||
| − | |style="background:LemonChiffon;"|10:20||align="center" style="background:LemonChiffon;"|SESSION 1 <br>Theory & Practice||align="center" style="background:LemonChiffon;"| Depth of Field<br>Depth of Focus ||align="center" style="background:LemonChiffon;"|PETER EVENNETT <br>LMF TEAM | + | |style="background:LemonChiffon;"|10:20||align="center" style="background:LemonChiffon;"|SESSION 1 <br>Theory & Practice||align="center" style="background:LemonChiffon;"| Depth of Field<br>Depth of Focus ||align="center" style="background:LemonChiffon;"|PETER EVENNETT <br>LMF TEAM || align="center" style="background:LemonChiffon;"| |
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| − | | 10:20||11:15||align="center"|SESSION 2<br>Theory & Demo||align="center" | Diffraction <br> Diffraction slide fun ||align="center" |PETER EVENNETT | + | | 10:20||11:15||align="center"|SESSION 2<br>Theory & Demo||align="center" | Diffraction <br> Diffraction slide fun ||align="center" |PETER EVENNETT || |
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| style="background:mediumseagreen;"|'''11:30''' | | style="background:mediumseagreen;"|'''11:30''' | ||
|align="center" style="background:mediumseagreen;"|'''BREAK''' | |align="center" style="background:mediumseagreen;"|'''BREAK''' | ||
| − | |align="center" style="background:mediumseagreen;"|'''COFFEE BREAK'''|| align="center" style="background:mediumseagreen;"|''Note: have break earlier next time, right after diffraction-string-show <br> (should be shortly before 11am)<br>then do all the explanation of Abbe's Diffraction Exp after break'' | + | |align="center" style="background:mediumseagreen;"|'''COFFEE BREAK'''|| align="center" style="background:mediumseagreen;"|''Note: have break earlier next time, right after diffraction-string-show <br> (should be shortly before 11am)<br>then do all the explanation of Abbe's Diffraction Exp after break'' ||align="center" style="background:mediumseagreen;" | |
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| − | | 11:30||12:50||align="center"|DEMONSTRATION || align="center"|ABBE'S DIFFRACTION EXPERIMENT<br>Peter's Video (50min) <br> Dan's setup demonstration||align="center"|PETER EVENNETT<br>DAN | + | | 11:30||12:50||align="center"|DEMONSTRATION || align="center"|ABBE'S DIFFRACTION EXPERIMENT<br>Peter's Video (50min) <br> Dan's setup demonstration||align="center"|PETER EVENNETT<br>DAN || |
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| style="background:mediumseagreen;"|'''13:50''' | | style="background:mediumseagreen;"|'''13:50''' | ||
|align="center" style="background:mediumseagreen;"|'''BREAK''' | |align="center" style="background:mediumseagreen;"|'''BREAK''' | ||
| − | |align="center" style="background:mediumseagreen;"|'''LUNCH'''|| style="background:mediumseagreen;"|''' ''' | + | |align="center" style="background:mediumseagreen;"|'''LUNCH'''|| style="background:mediumseagreen;"|''' ''' ||align="center" style="background:mediumseagreen;" | |
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| − | | 13:50||14:05||align="center"| || align="center"|spontaneous discussion :-) || | + | | 13:50||14:05||align="center"| || align="center"|spontaneous discussion :-) || || |
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| − | | 14:05||14:55||align="center"| SESSION 3<br>Theory || align="center"|Lens aberrations and corrections <br> Objectives ||align="center" |PETER EVENNETT | + | | 14:05||14:55||align="center"| SESSION 3<br>Theory || align="center"|Lens aberrations and corrections <br> Objectives ||align="center" |PETER EVENNETT || |
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| style="background:mediumseagreen;"|'''15:10''' | | style="background:mediumseagreen;"|'''15:10''' | ||
|align="center" style="background:mediumseagreen;"|'''BREAK''' | |align="center" style="background:mediumseagreen;"|'''BREAK''' | ||
| − | |align="center" style="background:mediumseagreen;"|'''COFFEE BREAK'''|| style="background:mediumseagreen;"|''' ''' | + | |align="center" style="background:mediumseagreen;"|'''COFFEE BREAK'''|| style="background:mediumseagreen;"|''' ''' ||align="center" style="background:mediumseagreen;" | |
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| − | | 15:10 || 15:55 ||align="center"|SESSION 4<br>Theory|| align="center"| Dark Field microscopy||align="center" |PETER EVENNETT | + | | 15:10 || 15:55 ||align="center"|SESSION 4<br>Theory|| align="center"| Dark Field microscopy||align="center" |PETER EVENNETT || |
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|style="background:LemonChiffon;"| 15:55 | |style="background:LemonChiffon;"| 15:55 | ||
| − | |style="background:LemonChiffon;"| 16:30 ||align="center" style="background:LemonChiffon;"|SESSION 4<br>Practice|| align="center" style="background:LemonChiffon;"|Dark Field microscopy||align="center" style="background:LemonChiffon;"|LMF TEAM | + | |style="background:LemonChiffon;"| 16:30 ||align="center" style="background:LemonChiffon;"|SESSION 4<br>Practice|| align="center" style="background:LemonChiffon;"|Dark Field microscopy||align="center" style="background:LemonChiffon;"|LMF TEAM || align="center" style="background:LemonChiffon;"| |
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| − | | 16:30||16:50||align="center"|SESSION 5<br>Theory|| align="center"|Basics of Phase Contrast microscopy ||align="center" |PETER EVENNETT | + | | 16:30||16:50||align="center"|SESSION 5<br>Theory|| align="center"|Basics of Phase Contrast microscopy ||align="center" |PETER EVENNETT || |
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|style="background:LemonChiffon;"| 16:50 | |style="background:LemonChiffon;"| 16:50 | ||
| − | |style="background:LemonChiffon;"|17:30||align="center" style="background:LemonChiffon;"|SESSION 5<br>Practice|| align="center" style="background:LemonChiffon;"|Phase Contrast microscopy ||align="center" style="background:LemonChiffon;"|LMF TEAM | + | |style="background:LemonChiffon;"|17:30||align="center" style="background:LemonChiffon;"|SESSION 5<br>Practice|| align="center" style="background:LemonChiffon;"|Phase Contrast microscopy ||align="center" style="background:LemonChiffon;"|LMF TEAM || align="center" style="background:LemonChiffon;"| |
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| − | | 17:30|| : ||align="center"|SESSION 5<br>Theory|| align="center"|Details of Phase Contrast microscopy ||align="center" |PETER EVENNETT | + | | 17:30|| : ||align="center"|SESSION 5<br>Theory|| align="center"|Details of Phase Contrast microscopy ||align="center" |PETER EVENNETT || |
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=== DAY THREE (May 18th)=== | === DAY THREE (May 18th)=== | ||
Revision as of 15:20, 24 October 2011
Contents |
PhD Program: Basics of Light Microscopy - Peter Evennett / LMF - Course May 2011
Setup -Fri 21 Oct
Setup - all lmf staff move stuff to galleria Equipment needed
- Microscopes
- Optical bench
- Spinning disk gadget
- Polarazing sheets
- Diffraction Grids
- Fluorescent samples
- Fluorescent Beads (Prepare slides)
- First day Handout for students
- Light torches
- cameras and monitors
- Cleaning stations for each table
- condenser screwdrivers for each microsocpe
- oil/DIC objectives for each microscope (next to stand)
- 2 polarization filters for each microscope
DAY ONE (24th Oct)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) | Materials needed |
| 9:00 | 09:30 | FINAL PREP | Test everything works | LMF | |
| 9:40 | 10:25 | INTRODUCTION | COURSE INTRO WHO is WHO? Round table |
LMF TEAM | White board and pen |
| 10:25 | 10:55 | Session 1 Theory |
PRINCIPLES OF LIGHT MICROSCOPY Historical aspects Very basic components and principles ( incl. Refraction-basics) |
PETER EVENNETT | |
| 10:55 | 11:10 | BREAK | BREAK | ||
| 11:15 | 11:30 | SESSION 1 Practice SHORT (5min !!!) Rotation |
capillary in oil demo Coin-in-cup demo red and green laser through milky water with black background |
DAN PETER PETE & JAN |
*capillaries, oil * coin-in-cup, water *laser pointer, glass with "milky" water and black background |
| 11:30 | 12:45 | SESSION 1 Theory |
Introduction to lenses (Lens demo fun) Lens aberrations (see colour fringes of lighting image with simple lens) Resolution, Numerical aperture Magnification |
PETER EVENNETT | little lenses |
| 12:45 | 13:45 | LUNCH | LUNCH | ||
| 13:45 | 14:45 | SESSION 1 Practice 15min (!!!) Rotation |
Milky Glass Block demo Show and discuss microscope parts (upright and inverted) NA measurements |
DAN JAN & BRITTA SILKE |
*milky glass block, teaching microscope, coloured filters for microscope, iris objective *slides with arrows, simple upright and inverted microscope *horizontal protractor on stand, objective holder, 2 air objective with different (!) NAs (and maybe an iris objective), calculator, pencils, rubber |
| 14:45 | 15:30 | SESSION 2 Theory |
Microscope illumination Conjugate planes |
PETER EVENNETT | |
| 15:30 | 15:45 | BREAK | BREAK | ||
| 15:45 | 16:50 | SESSION 2 Practice (30min Rotation) |
conjugate planes on Peters microscope conjugate planes on optical bench |
PETER EVENNETT JAN |
*Peters microscope setup *optical bench demonstrating a transmitted light microscope, incl. light source |
| 16:50 | 17:15 | SESSION 3 Theory |
Koehler Illumination (Microscope alignment) |
PETER EVENNETT | |
| 17:15 | 18:30 | SESSION 3 Practice |
Koehler illumination (Microscope alignment) |
LMF TEAM | students manual microscopes, screwdrivers, nice brightfield sample |
DAY TWO (May 17th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) | Materials needed |
| 9:00 | 10:00 | Recap | day 1 | LMF TEAM | |
| 10:00 | 10:20 | SESSION 1 Theory & Practice |
Depth of Field Depth of Focus |
PETER EVENNETT LMF TEAM |
|
| 10:20 | 11:15 | SESSION 2 Theory & Demo |
Diffraction Diffraction slide fun |
PETER EVENNETT | |
| 11:15 | 11:30 | BREAK | COFFEE BREAK | Note: have break earlier next time, right after diffraction-string-show (should be shortly before 11am) then do all the explanation of Abbe's Diffraction Exp after break |
|
| 11:30 | 12:50 | DEMONSTRATION | ABBE'S DIFFRACTION EXPERIMENT Peter's Video (50min) Dan's setup demonstration |
PETER EVENNETT DAN |
|
| 12:50 | 13:50 | BREAK | LUNCH | ||
| 13:50 | 14:05 | spontaneous discussion :-) | |||
| 14:05 | 14:55 | SESSION 3 Theory |
Lens aberrations and corrections Objectives |
PETER EVENNETT | |
| 14:55 | 15:10 | BREAK | COFFEE BREAK | ||
| 15:10 | 15:55 | SESSION 4 Theory |
Dark Field microscopy | PETER EVENNETT | |
| 15:55 | 16:30 | SESSION 4 Practice |
Dark Field microscopy | LMF TEAM | |
| 16:30 | 16:50 | SESSION 5 Theory |
Basics of Phase Contrast microscopy | PETER EVENNETT | |
| 16:50 | 17:30 | SESSION 5 Practice |
Phase Contrast microscopy | LMF TEAM | |
| 17:30 | : | SESSION 5 Theory |
Details of Phase Contrast microscopy | PETER EVENNETT |
DAY THREE (May 18th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) | Materials needed |
| 9:00 | 10:00 | Recap | day 2 | LMF TEAM | |
| 10:00 | 10:20 | SESSION 1 Theory |
Objective reading | PETER EVENNETT LMF TEAM | |
| 10:20 | 10:35 | BREAK | BREAK | BREAK | |
| 10:40 | 12:10 | SESSION 1 Practice Group rotation (30min. each!) |
Cover glasses and sample mounting Objective reading Objective cleaning |
Dan Britta, Silke Jan | |
| 12:10 | 13:00 | SESSION 2 Theory |
Polarized light microscopy | PETER EVENNETT | |
| 13:00 | 14:00 | BREAK | LUNCH | ||
| 14:00 | 14:40 | SESSION 2 Practice |
Polarized light microscopy | LMF TEAM | |
| 14:40 | 15:05 | SESSION 3 Theory |
Differential Interference Contrast (DIC) | PETER EVENNETT | |
| 15:05 | 15:40 | SESSION 3 Practice |
Differential Interference Contrast (DIC) | LMF TEAM | |
| 15:40 | 16:00 | BREAK | BREAK | BREAK | |
| 16:00 | 16:30 | Question round | Questions to Peter Evennett | PETER EVENNETT LMF TEAM | |
| 16:30 | 17:40 | SESSION 4 Theory & Demo |
What is a pixel Analog to Digital conversion Nuyquist-Shannon |
Dan | |
| 17:40 | 18:00 | SESSION 3 Practice |
PIXELS Sesame seeds, sunflower seeds, nudels, gumibears and a checker board |
LMF TEAM |
DAY FOUR (May 19th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) | DEMO Activities | Materials needed |
| 9:00 | 10:00 | Recap | day 3 | LMF TEAM | ||
| 10:00 | 10:45 | SESSION 1 Theory |
Fundamental concepts of fluorescence | DAN | - | - |
| 10:45 | 11:00 | BREAK | BREAK | BREAK | BREAK | BREAK |
| 11:00 | 12:15 | SESSION 2 Theory & Practice |
Fluorescence Microscopy | SILKE LMFTEAM |
• Light sources • Lamp houses • Spectra • Filters • Filter cubes • Filter spectral charts of each microscope (laminated) |
• Spare lamp houses • Lamps • Ocean optics equipment • Four (4) different Filter-sets (cubes), with the appropriate descriptions • diagramms for drawing filter/fluorophore spectra |
| 12:15 | 13:00 | SESSION 2 Practice |
Fluorescence imaging at microscopes | LMF TEAM | - | • Fluorescent labeled samples |
| 13:00 | 14:00 | BREAK | LUNCH | LUNCH | LUNCH | LUNCH |
| 14:00 | 15:45 | SESSION 3 Theory & Practice |
Imaging with CCD and Quantitative Imaging | LMF TEAM Britta/Dan |
• CCD • Cameras |
• CCD chip • RGB Slider Spot Camera setup |
| 15:45 | 16:00 | BREAK | BREAK | |||
| 16:00 | 18:00 | SESSION 4 Practice |
Imaging with CCD cameras using LMF systems | LMF TEAM | • Displaying • Pixel size • Look up table • Binning • Exposure time • Saturation • Signal to noise • ROI • Bit depth • Auto map • Auto contrast |
• Microscopes: DVcore(Dan, 3 students) pDV (Silke, 2 students) Inverted CCD (Pete, 2 students) N-Storm (Britta, 2 students) self-built-syst (Jan, 3 students)) • Fluorescence Sample slides (Kidney / Cells) |
| 19:00 | open end | Dinner | with Peter | LMF TEAM |
DAY FIVE (May 20th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) | Materials needed |
| 9:00 | 10:00 | Recap | day 4 | LMF TEAM | |
| 10:00 | 11:10 | SESSION 1 Theory & Demo |
Optical sectioning methods Pros&Cons |
LMF TEAM Dan / Jan | |
| 11:10 | 11:25 | BREAK | BREAK | BREAK | |
| 11:25 | 13:00 | SESSION 1 Theory & Demo |
Optical sectioning methods Pros&Cons |
LMF TEAM Dan / Jan | |
| 13:00 | 14:00 | BREAK | LUNCH | ||
| 14:00 | 15:00 | SESSION 2 Small Groups Discussion |
What techniques might be good for me (Own Projects) |
LMF TEAM Dan/Jan/Silke/Britta/Pete? | |
| 15:00 | 15:15 | BREAK | BREAK | BREAK | |
| 15:15 | 16:00 | SESSION 3 | Course evaluation | LMF TEAM |
