CL1 - Leica TCS SP2
From BioDIP
(Difference between revisions)
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|facility_image=logo-lmf.jpg | |facility_image=logo-lmf.jpg | ||
|description=[[Leica TCS SP2]] single photon [[confocal]] scanning system with three confocal PMTs and transmitted light detector. | |description=[[Leica TCS SP2]] single photon [[confocal]] scanning system with three confocal PMTs and transmitted light detector. | ||
| − | |applications=experiments and samples that require dipping lenses | + | |applications=experiments and samples that require dipping lenses<br><br>[[Leica TCS SP2 - Beam Expander|recommended 'beam expander' settings]] |
|image=MPI03.jpg | |image=MPI03.jpg | ||
|stand=Upright | |stand=Upright | ||
|microscope=manual XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with manual DIC | |microscope=manual XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with manual DIC | ||
| − | |objectives=10x/0.3 Fluotar<br>20x/0.7 Imm corr CS Apo<br>40x/1.25-0.75 Oil CS Apo<br>63x/1.32-0.6 Oil CS Apo<br>63x/0.9 W Dipping Apo | + | |objectives=10x/0.3 Fluotar<br>20x/0.7 Imm corr CS Apo<br>40x/1.25-0.75 Oil CS Apo<br>63x/1.32-0.6 Oil CS Apo<br>63x/0.9 W Dipping Apo |
|illumination=Halogen<br>HBO<br>458, 476, 488, 514 nm Argon laser<br>543 nm HeNe laser<br>633 nm HeNe laser | |illumination=Halogen<br>HBO<br>458, 476, 488, 514 nm Argon laser<br>543 nm HeNe laser<br>633 nm HeNe laser | ||
|detection=point scanner, 3 confocal PMTs, T-PMT, prism and spectral sliders for detection range selection, central pinhole | |detection=point scanner, 3 confocal PMTs, T-PMT, prism and spectral sliders for detection range selection, central pinhole | ||
Revision as of 17:24, 1 September 2009
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , Upright stand, manual XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with manual DIC | ||
| objectives | |||
| illumination |
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| detection | point scanner, 3 confocal PMTs, T-PMT, prism and spectral sliders for detection range selection, central pinhole | ||
| reflectors | EX 450-490, EM LP515 EX 515-560, EM LP590 | ||
| features | imaging up to 4096x4096, simultaneous imaging up to 3 channels, sequential imaging, spectral imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation | ||
| software | |||
| incubation | - | ||
| links |
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| inv.nr. | 402527 |
