CZ2 - Zeiss LSM UV

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{{Equipment
 
{{Equipment
| system-number       = MPI02
+
|system-number=MPI-CZ2
| system-name         = Zeiss LSM UV
+
|system-name=Zeiss LSM UV
| category             = Laser Scanning Confocal
+
|type=Not listed
| stand                = inverted
+
|category=Laser Scanning Confocal
| image                = MPI02.jpg
+
|facility=MPI-CBG LMF
| facility            = MPI-CBG LMF
+
|building=MPI-CBG
| facility_image      = logo-lmf.jpg
+
|room=038A
| building            = MPI-CBG
+
|Facility-logo=MPI-CBG-Lmf-logo-big.png
| room                = 038A
+
|Institute-logo=Logo MPI-CBG.jpg
| description         = Single photon [[Zeiss LSM 510]] [[confocal]] system with three PMTs and transmitted light detector. Inverted setup with UV laser.
+
|description=Single photon [[Zeiss LSM 510]] [[confocal]] system with three PMTs and transmitted light detector. Inverted setup with UV laser.
| applications         = for routine dye combinations as well as special stainings that require UV excitation, i.e. lipid dyes.
+
|applications=for routine dye combinations as well as special stainings that require UV excitation, i.e. lipid dyes.
| microscope          = inverted stand, manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC  
+
|image=CZ2 - Zeiss LSM UV.jpg
| objectives          = 10x/0.45 Apo<br>25x/0.8 ImmCorr<br>40x/1.3 Oil<br>63x/1.4 Oil Apo<br>63x/1.2 W Apo<br>100x/1.3 Oil
+
|stand-name=Zeiss - Axiovert 200M
| illumination        = Halogen<br>HBO<br>351, 364 nm UV laser<br>458, 477, 488, 514 nm Argon laser<br>543 nm HeNe laser<br>633 nm HeNe laser
+
|stand=Inverted
| detection           = point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector
+
|microscope=manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC. Axiovert 200 M 1005-820 SN: 000181  Baujahr 2001.
| reflectors           = EX 359-371, EM LP397<br>EX 449-498, EM LP520<br>EX 540-551, EM LP590
+
|obj1=Zeiss Plan-Apochromat 10x 0.45
| features             = simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation
+
|obj2=Zeiss Plan-Neofluar 25x 0.8 Oil/Gly/Water
| incubation           = mobile stage incubator
+
|obj3=Zeiss Plan Neofluar 40x 1.3 Oil
| link1               = [http://spreadsheets.google.com/pub?key=pFACvabR4mSk0c_q3sEgGIw laser power]
+
|obj4=Zeiss Plan-Neofluar 100x 1.3 Oil
| link2               = [http://spreadsheets.google.com/pub?key=pMZJXCgd5HNsP92avQu4iGA scan-head configuration]
+
|obj5=Zeiss Plan-Apochromat 63x 1.4 Oil
| inv                 = 2002-01425 SIP: 71648  
+
|ill1=Transmitted Light (Halogen)
 +
|ill2=Fluorescence (Metal Halide, X-Cite, 120W)
 +
|ill3=Laser UV 351, 364 nm
 +
|ill4=Laser Argon Multiline 458, 477, 488, 514 nm
 +
|ill5=Laser HeNe 543 nm
 +
|ill6=Laser HeNe 633 nm
 +
|detection=point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector
 +
|reflectors=EX 359-371, EM LP397 (Zeiss FS01)<br>EX 449-498, EM LP520 (Zeiss FS09)<br>EX 540-551, EM LP590 (Zeiss FS15)
 +
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation
 +
|software=-
 +
|incubation=Stage incubator (T) + objective heater
 +
|link0=https://ifn.mpi-cbg.de/wiki/MPI-CZ2_-_Zeiss_LSM_UV_-_startup_%26_shutdown
 +
|link1=http://spreadsheets.google.com/pub?key=pFACvabR4mSk0c_q3sEgGIw
 +
|link2=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNsP92avQu4iGA
 +
|inv=2002-01425 SIP: 71648 10.1.34.217
 
}}
 
}}

Latest revision as of 15:42, 3 February 2016

Suitable for for routine dye combinations as well as special stainings that require UV excitation, i.e. lipid dyes..
CZ2 - Zeiss LSM UV.jpg


[edit] Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 038A)

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope Zeiss - Axiovert 200M, Inverted stand, manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC. Axiovert 200 M 1005-820 SN: 000181 Baujahr 2001.
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Neofluar 25x 0.8 Oil/Gly/Water
Zeiss Plan Neofluar 40x 1.3 Oil
Zeiss Plan-Neofluar 100x 1.3 Oil
Zeiss Plan-Apochromat 63x 1.4 Oil
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide, X-Cite, 120W)
Laser UV 351, 364 nm
Laser Argon Multiline 458, 477, 488, 514 nm
Laser HeNe 543 nm
Laser HeNe 633 nm
detection point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector
reflectors EX 359-371, EM LP397 (Zeiss FS01)
EX 449-498, EM LP520 (Zeiss FS09)
EX 540-551, EM LP590 (Zeiss FS15)
features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation
software -
incubation Stage incubator (T) + objective heater
links
inv.nr. 2002-01425 SIP: 71648 10.1.34.217



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