CZ2 - Zeiss LSM UV
From BioDIP
(Difference between revisions)
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{{Equipment | {{Equipment | ||
− | | system-number | + | |system-number=MPI02 |
− | | system-name | + | |system-name=Zeiss LSM UV |
− | | category | + | |type=Microscope |
− | + | |category=Laser Scanning Confocal | |
− | + | |facility=MPI-CBG LMF | |
− | | facility | + | |building=MPI-CBG |
− | + | |room=038A | |
− | | building | + | |facility_image=logo-lmf.jpg |
− | | room | + | |description=Single photon [[Zeiss LSM 510]] [[confocal]] system with three PMTs and transmitted light detector. Inverted setup with UV laser. |
− | | description | + | |applications=for routine dye combinations as well as special stainings that require UV excitation, i.e. lipid dyes. |
− | | applications | + | |image=MPI02.jpg |
− | | microscope | + | |stand=3D |
− | | objectives | + | |microscope=manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC |
− | | illumination | + | |objectives=10x/0.45 Apo<br>25x/0.8 ImmCorr<br>40x/1.3 Oil<br>63x/1.4 Oil Apo<br>63x/1.2 W Apo<br>100x/1.3 Oil |
− | | detection | + | |illumination=Halogen<br>HBO<br>351, 364 nm UV laser<br>458, 477, 488, 514 nm Argon laser<br>543 nm HeNe laser<br>633 nm HeNe laser |
− | | reflectors | + | |detection=point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector |
− | | features | + | |reflectors=EX 359-371, EM LP397 (Zeiss FS01)<br>EX 449-498, EM LP520 (Zeiss FS09)<br>EX 540-551, EM LP590 (Zeiss FS15) |
− | | incubation | + | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation |
− | | link1 | + | |incubation=stage incubator (T) + objective heater |
− | | link2 | + | |link1=[http://spreadsheets.google.com/pub?key=pFACvabR4mSk0c_q3sEgGIw laser power] |
− | | inv | + | |link2=[http://spreadsheets.google.com/pub?key=pMZJXCgd5HNsP92avQu4iGA scan-head configuration] |
+ | |inv=2002-01425 SIP: 71648 | ||
+ | |obj1=Empty position | ||
+ | |obj2=Empty position | ||
+ | |obj3=Empty position | ||
+ | |obj4=Empty position | ||
+ | |obj5=Empty position | ||
+ | |obj6=Empty position | ||
+ | |obj7=Empty position | ||
+ | |obj8=Empty position | ||
}} | }} |
Revision as of 16:55, 1 September 2009
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | , 3D stand, manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
objectives | |||
illumination |
| ||
detection | point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
reflectors | EX 359-371, EM LP397 (Zeiss FS01) EX 449-498, EM LP520 (Zeiss FS09) EX 540-551, EM LP590 (Zeiss FS15) | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation | ||
software | |||
incubation | stage incubator (T) + objective heater | ||
links |
| ||
inv.nr. | 2002-01425 SIP: 71648 |