| system-number       = 04

| system-name         = Zeiss LSM 405/594

| category             = Laser Scanning Confocal

| stand                = inverted

| image                = MPI04.jpg

| facility            = MPI-CBG LMF

| facility_image      = logo-lmf.jpg

| building            = MPI-CBG

| description         = Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs and transmitted light detector.  

| applications         = dual color imaging of EGFP + mCherry

| microscope           = inverted stand, manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC

| objectives          = 10x/0.45 Plan-Neo<br>25x/0.8 Imm corr. Plan-Neo<br>40x/1.3 Oil Plan-Neo<br>40x/1.2 W corr. Apo<br>63x/1.4 Oil Plan-Apo<br>63x/1.2 W Apo

| illumination        = Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>594 nm HeNe laser<br>633 nm HeNe laser

| detection           = point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector

| reflectors           = EX 323-380, EM 417-469<br>EX 422-443, EM 458-498<br>EX 451-489, EM 496-546<br>EX 531-581, EM LP617

| features             = simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, [[averaging]], summarizing, scan zoom and rotation

| incubation           = mobile stage incubator

| link1               = [http://spreadsheets.google.com/pub?key=pFACvabR4mSlISzehf0x6JQ LPM]

| link2               = [http://web-redaktion.tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging/ifn/equipment-files/04_zeiss_lsm_405-594_filterconfig.pdf scan-head filter configuration]

| inv                  =