CZ3 - Zeiss LSM 405/594
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|system-number=MPI-CZ3 | |system-number=MPI-CZ3 | ||
|system-name=Zeiss LSM 405/594 | |system-name=Zeiss LSM 405/594 | ||
| − | |type= | + | |type=Not listed |
|category=Laser Scanning Confocal | |category=Laser Scanning Confocal | ||
|facility=MPI-CBG LMF | |facility=MPI-CBG LMF | ||
|building=MPI-CBG | |building=MPI-CBG | ||
|room=038A | |room=038A | ||
| − | | | + | |Facility-logo=MPI-CBG-Lmf-logo-big.png |
| + | |Institute-logo=Logo MPI-CBG.jpg | ||
|description=Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs and transmitted light detector. | |description=Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs and transmitted light detector. | ||
|applications=dual color imaging of EGFP + mCherry and routine confocal imaging of suitable dye combinations | |applications=dual color imaging of EGFP + mCherry and routine confocal imaging of suitable dye combinations | ||
|image=MPI04.jpg | |image=MPI04.jpg | ||
| + | |stand-name=Zeiss - Axiovert 200M | ||
|stand=Inverted | |stand=Inverted | ||
|microscope=inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | |microscope=inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
|obj1=Zeiss Plan-Apochromat 10x 0.45 | |obj1=Zeiss Plan-Apochromat 10x 0.45 | ||
| − | |obj2=Zeiss | + | |obj2=Zeiss Plan-Neofluar 25x 0.8 Oil/Gly/Water |
| − | |obj3=Zeiss | + | |obj3=Zeiss Plan Neofluar 40x 1.3 Oil |
| − | |obj4=Zeiss | + | |obj4=Zeiss Plan-Apochromat 63x 1.4 Oil |
| − | |obj5=Zeiss | + | |obj5=Zeiss C-Apochromat 40x 1.2 W |
|obj6=Zeiss C-Apochromat 63x 1.2 W | |obj6=Zeiss C-Apochromat 63x 1.2 W | ||
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
| − | |ill2=Fluorescence ( | + | |ill2=Fluorescence (Metal Halide, X-Cite, 120W) |
|ill3=Laser Diode 405 nm | |ill3=Laser Diode 405 nm | ||
|ill4=Laser Argon Multiline 458, 477, 488, 514 nm | |ill4=Laser Argon Multiline 458, 477, 488, 514 nm | ||
| Line 28: | Line 30: | ||
|reflectors=EX 323-380, EM 417-469 (Zeiss FS00wf)<br>EX 422-443, EM 458-498 (Zeiss FS38wf)<br>EX 451-489, EM 496-546 (Zeiss FS47wf)<br>EX 531-581, EM LP617 (Zeiss FS49) | |reflectors=EX 323-380, EM 417-469 (Zeiss FS00wf)<br>EX 422-443, EM 458-498 (Zeiss FS38wf)<br>EX 451-489, EM 496-546 (Zeiss FS47wf)<br>EX 531-581, EM LP617 (Zeiss FS49) | ||
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan | ||
| + | |software=- | ||
|incubation=Stage incubator (T) + objective heater | |incubation=Stage incubator (T) + objective heater | ||
| + | |link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-CZ3_-_Zeiss_LSM_405/594_-_startup_%26_shutdown | ||
|link1=http://spreadsheets.google.com/pub?key=pFACvabR4mSlISzehf0x6JQ | |link1=http://spreadsheets.google.com/pub?key=pFACvabR4mSlISzehf0x6JQ | ||
|link2=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNstvdFBlQXDNw | |link2=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNstvdFBlQXDNw | ||
| − | |inv=404687 SIP:71680 | + | |inv=404687 SIP:71680 10.1.15.75 10.1.39.223 |
| + | |facility_image=logo-lmf.jpg | ||
|objectives=10x/0.45 Plan-Neo<br>25x/0.8 Imm corr. Plan-Neo<br>40x/1.3 Oil Plan-Neo<br>40x/1.2 W corr. Apo<br>63x/1.4 Oil Plan-Apo<br>63x/1.2 W Apo | |objectives=10x/0.45 Plan-Neo<br>25x/0.8 Imm corr. Plan-Neo<br>40x/1.3 Oil Plan-Neo<br>40x/1.2 W corr. Apo<br>63x/1.4 Oil Plan-Apo<br>63x/1.2 W Apo | ||
|illumination=Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>594 nm HeNe laser<br>633 nm HeNe laser | |illumination=Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>594 nm HeNe laser<br>633 nm HeNe laser | ||
}} | }} | ||
Latest revision as of 15:43, 3 February 2016
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[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
| microscope | Zeiss - Axiovert 200M, Inverted stand, inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
| objectives | |||
| illumination | |||
| detection | point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
| reflectors | EX 323-380, EM 417-469 (Zeiss FS00wf) EX 422-443, EM 458-498 (Zeiss FS38wf) EX 451-489, EM 496-546 (Zeiss FS47wf) EX 531-581, EM LP617 (Zeiss FS49) | ||
| features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan | ||
| software | - | ||
| incubation | Stage incubator (T) + objective heater | ||
| links |
| ||
| inv.nr. | 404687 SIP:71680 10.1.15.75 10.1.39.223"404687 SIP:71680 10.1.15.75 10.1.39.223" cannot be used as a page name in this wiki. |
