CZ3 - Zeiss LSM 405/594

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|system-number=MPI-CZ3
 
|system-number=MPI-CZ3
 
|system-name=Zeiss LSM 405/594
 
|system-name=Zeiss LSM 405/594
|type=Microscope
+
|type=Not listed
 
|category=Laser Scanning Confocal
 
|category=Laser Scanning Confocal
 
|facility=MPI-CBG LMF
 
|facility=MPI-CBG LMF
 
|building=MPI-CBG
 
|building=MPI-CBG
 
|room=038A
 
|room=038A
 +
|Facility-logo=MPI-CBG-Lmf-logo-big.png
 +
|Institute-logo=Logo MPI-CBG.jpg
 
|description=Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs and transmitted light detector.
 
|description=Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs and transmitted light detector.
 
|applications=dual color imaging of EGFP + mCherry and routine confocal imaging of suitable dye combinations
 
|applications=dual color imaging of EGFP + mCherry and routine confocal imaging of suitable dye combinations
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|microscope=inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
 
|microscope=inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
 
|obj1=Zeiss Plan-Apochromat 10x 0.45
 
|obj1=Zeiss Plan-Apochromat 10x 0.45
|obj2=Zeiss Plan-Neofluar 25x 0.8 Imm Corr
+
|obj2=Zeiss Plan-Neofluar 25x 0.8 Oil/Gly/Water
|obj3=Zeiss Plan Neofluar 40x 1.3 oil
+
|obj3=Zeiss Plan Neofluar 40x 1.3 Oil
 
|obj4=Zeiss Plan-Apochromat 63x 1.4 Oil
 
|obj4=Zeiss Plan-Apochromat 63x 1.4 Oil
 
|obj5=Zeiss C-Apochromat 40x 1.2 W
 
|obj5=Zeiss C-Apochromat 40x 1.2 W
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|link1=http://spreadsheets.google.com/pub?key=pFACvabR4mSlISzehf0x6JQ
 
|link1=http://spreadsheets.google.com/pub?key=pFACvabR4mSlISzehf0x6JQ
 
|link2=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNstvdFBlQXDNw
 
|link2=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNstvdFBlQXDNw
|inv=404687 SIP:71680
+
|inv=404687 SIP:71680 10.1.15.75 10.1.39.223
 
|facility_image=logo-lmf.jpg
 
|facility_image=logo-lmf.jpg
 
|objectives=10x/0.45 Plan-Neo<br>25x/0.8 Imm corr. Plan-Neo<br>40x/1.3 Oil Plan-Neo<br>40x/1.2 W corr. Apo<br>63x/1.4 Oil Plan-Apo<br>63x/1.2 W Apo
 
|objectives=10x/0.45 Plan-Neo<br>25x/0.8 Imm corr. Plan-Neo<br>40x/1.3 Oil Plan-Neo<br>40x/1.2 W corr. Apo<br>63x/1.4 Oil Plan-Apo<br>63x/1.2 W Apo
 
|illumination=Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>594 nm HeNe laser<br>633 nm HeNe laser
 
|illumination=Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>594 nm HeNe laser<br>633 nm HeNe laser
 
}}
 
}}

Latest revision as of 15:43, 3 February 2016

Suitable for dual color imaging of EGFP + mCherry and routine confocal imaging of suitable dye combinations.
MPI04.jpg


[edit] Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 038A)

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope Zeiss - Axiovert 200M, Inverted stand, inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Neofluar 25x 0.8 Oil/Gly/Water
Zeiss Plan Neofluar 40x 1.3 Oil
Zeiss Plan-Apochromat 63x 1.4 Oil
Zeiss C-Apochromat 40x 1.2 W
Zeiss C-Apochromat 63x 1.2 W
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide, X-Cite, 120W)
Laser Diode 405 nm
Laser Argon Multiline 458, 477, 488, 514 nm
Laser HeNe 594 nm
Laser HeNe 633 nm
detection point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector
reflectors EX 323-380, EM 417-469 (Zeiss FS00wf)
EX 422-443, EM 458-498 (Zeiss FS38wf)
EX 451-489, EM 496-546 (Zeiss FS47wf)
EX 531-581, EM LP617 (Zeiss FS49)
features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan
software -
incubation Stage incubator (T) + objective heater
links
inv.nr. 404687 SIP:71680 10.1.15.75 10.1.39.223"404687 SIP:71680 10.1.15.75 10.1.39.223" cannot be used as a page name in this wiki.



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