CZ3 - Zeiss LSM 405/594

From BioDIP
(Difference between revisions)
Jump to: navigation, search
(New page: == [{{{system-number}}}] {{{system-name}}} == ---- {| style="width:100%" |-valign="top" |width=90%| {{{category}}}<br>{{{building}}}, {{{room}}}<br><br>''{{{description}}}'' |width=200px| ...)
 
 
(38 intermediate revisions by 4 users not shown)
Line 1: Line 1:
−
== [{{{system-number}}}] {{{system-name}}} ==
+
{{Equipment
−
----
+
|system-number=MPI-CZ3
−
{| style="width:100%"
+
|system-name=Zeiss LSM 405/594
−
|-valign="top"
+
|type=Not listed
−
|width=90%| {{{category}}}<br>{{{building}}}, {{{room}}}<br><br>''{{{description}}}''
+
|category=Laser Scanning Confocal
−
|width=200px| [[image:{{{image}}}|200px]]
+
|facility=MPI-CBG LMF
−
|}
+
|building=MPI-CBG
−
 
+
|room=038A
−
 
+
|Facility-logo=MPI-CBG-Lmf-logo-big.png
−
{| style="width:100%"
+
|Institute-logo=Logo MPI-CBG.jpg
−
|-valign="top"
+
|description=Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs and transmitted light detector.
−
|width=120px| '''microscope'''
+
|applications=dual color imaging of EGFP + mCherry and routine confocal imaging of suitable dye combinations
−
| {{{microscope}}}
+
|image=MPI04.jpg
−
|-valign="top"
+
|stand-name=Zeiss - Axiovert 200M
−
|width=120px| '''objectives'''
+
|stand=Inverted
−
| {{{objectives}}}
+
|microscope=inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
−
|-valign="top"
+
|obj1=Zeiss Plan-Apochromat 10x 0.45
−
|width=120px| '''excitation'''
+
|obj2=Zeiss Plan-Neofluar 25x 0.8 Oil/Gly/Water
−
| {{{excitation}}}
+
|obj3=Zeiss Plan Neofluar 40x 1.3 Oil
−
|-valign="top"
+
|obj4=Zeiss Plan-Apochromat 63x 1.4 Oil
−
|width=120px| '''detection'''
+
|obj5=Zeiss C-Apochromat 40x 1.2 W
−
| {{{detection}}}
+
|obj6=Zeiss C-Apochromat 63x 1.2 W
−
|-valign="top"
+
|ill1=Transmitted Light (Halogen)
−
|width=120px| '''reflectors'''
+
|ill2=Fluorescence (Metal Halide, X-Cite, 120W)
−
| {{{reflectors}}}
+
|ill3=Laser Diode 405 nm
−
|-valign="top"
+
|ill4=Laser Argon Multiline 458, 477, 488, 514 nm
−
|width=120px| '''features'''
+
|ill5=Laser HeNe 594 nm
−
| {{{features}}}
+
|ill6=Laser HeNe 633 nm
−
|-valign="top"
+
|detection=point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector
−
|width=120px| '''incubation'''
+
|reflectors=EX 323-380, EM 417-469 (Zeiss FS00wf)<br>EX 422-443, EM 458-498 (Zeiss FS38wf)<br>EX 451-489, EM 496-546 (Zeiss FS47wf)<br>EX 531-581, EM LP617 (Zeiss FS49)
−
| {{{incubation}}}
+
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan
−
|}
+
|software=-
−
<br>
+
|incubation=Stage incubator (T) + objective heater
−
----
+
|link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-CZ3_-_Zeiss_LSM_405/594_-_startup_%26_shutdown
−
{| style="width:100%"
+
|link1=http://spreadsheets.google.com/pub?key=pFACvabR4mSlISzehf0x6JQ
−
|width=95%|
+
|link2=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNstvdFBlQXDNw
−
| [[image:{{{facility_image}}}|100px|right]]
+
|inv=404687 SIP:71680  10.1.15.75 10.1.39.223
−
|}
+
|facility_image=logo-lmf.jpg
−
[[Category:{{{category}}}]]
+
|objectives=10x/0.45 Plan-Neo<br>25x/0.8 Imm corr. Plan-Neo<br>40x/1.3 Oil Plan-Neo<br>40x/1.2 W corr. Apo<br>63x/1.4 Oil Plan-Apo<br>63x/1.2 W Apo
 +
|illumination=Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>594 nm HeNe laser<br>633 nm HeNe laser
 +
}}

Latest revision as of 15:43, 3 February 2016

Suitable for dual color imaging of EGFP + mCherry and routine confocal imaging of suitable dye combinations.
MPI04.jpg


[edit] Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 038A)

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope Zeiss - Axiovert 200M, Inverted stand, inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Neofluar 25x 0.8 Oil/Gly/Water
Zeiss Plan Neofluar 40x 1.3 Oil
Zeiss Plan-Apochromat 63x 1.4 Oil
Zeiss C-Apochromat 40x 1.2 W
Zeiss C-Apochromat 63x 1.2 W
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide, X-Cite, 120W)
Laser Diode 405 nm
Laser Argon Multiline 458, 477, 488, 514 nm
Laser HeNe 594 nm
Laser HeNe 633 nm
detection point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector
reflectors EX 323-380, EM 417-469 (Zeiss FS00wf)
EX 422-443, EM 458-498 (Zeiss FS38wf)
EX 451-489, EM 496-546 (Zeiss FS47wf)
EX 531-581, EM LP617 (Zeiss FS49)
features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan
software -
incubation Stage incubator (T) + objective heater
links
inv.nr. 404687 SIP:71680 10.1.15.75 10.1.39.223"404687 SIP:71680 10.1.15.75 10.1.39.223" cannot be used as a page name in this wiki.


Retrieved from "https://www.biodip.de/w/index.php?title=CZ3_-_Zeiss_LSM_405/594&oldid=11067"
Personal tools