CZ3 - Zeiss LSM 405/594
From BioDIP
(Difference between revisions)
(32 intermediate revisions by 4 users not shown) | |||
Line 1: | Line 1: | ||
{{Equipment | {{Equipment | ||
− | | system-number | + | |system-number=MPI-CZ3 |
− | | system-name | + | |system-name=Zeiss LSM 405/594 |
− | | category | + | |type=Not listed |
− | | | + | |category=Laser Scanning Confocal |
− | | | + | |facility=MPI-CBG LMF |
− | + | |building=MPI-CBG | |
− | | | + | |room=038A |
− | | | + | |Facility-logo=MPI-CBG-Lmf-logo-big.png |
− | + | |Institute-logo=Logo MPI-CBG.jpg | |
− | | description | + | |description=Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs and transmitted light detector. |
− | | microscope | + | |applications=dual color imaging of EGFP + mCherry and routine confocal imaging of suitable dye combinations |
− | | | + | |image=MPI04.jpg |
− | | | + | |stand-name=Zeiss - Axiovert 200M |
− | | detection | + | |stand=Inverted |
− | | reflectors | + | |microscope=inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC |
− | | features | + | |obj1=Zeiss Plan-Apochromat 10x 0.45 |
− | | incubation | + | |obj2=Zeiss Plan-Neofluar 25x 0.8 Oil/Gly/Water |
+ | |obj3=Zeiss Plan Neofluar 40x 1.3 Oil | ||
+ | |obj4=Zeiss Plan-Apochromat 63x 1.4 Oil | ||
+ | |obj5=Zeiss C-Apochromat 40x 1.2 W | ||
+ | |obj6=Zeiss C-Apochromat 63x 1.2 W | ||
+ | |ill1=Transmitted Light (Halogen) | ||
+ | |ill2=Fluorescence (Metal Halide, X-Cite, 120W) | ||
+ | |ill3=Laser Diode 405 nm | ||
+ | |ill4=Laser Argon Multiline 458, 477, 488, 514 nm | ||
+ | |ill5=Laser HeNe 594 nm | ||
+ | |ill6=Laser HeNe 633 nm | ||
+ | |detection=point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
+ | |reflectors=EX 323-380, EM 417-469 (Zeiss FS00wf)<br>EX 422-443, EM 458-498 (Zeiss FS38wf)<br>EX 451-489, EM 496-546 (Zeiss FS47wf)<br>EX 531-581, EM LP617 (Zeiss FS49) | ||
+ | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan | ||
+ | |software=- | ||
+ | |incubation=Stage incubator (T) + objective heater | ||
+ | |link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-CZ3_-_Zeiss_LSM_405/594_-_startup_%26_shutdown | ||
+ | |link1=http://spreadsheets.google.com/pub?key=pFACvabR4mSlISzehf0x6JQ | ||
+ | |link2=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNstvdFBlQXDNw | ||
+ | |inv=404687 SIP:71680 10.1.15.75 10.1.39.223 | ||
+ | |facility_image=logo-lmf.jpg | ||
+ | |objectives=10x/0.45 Plan-Neo<br>25x/0.8 Imm corr. Plan-Neo<br>40x/1.3 Oil Plan-Neo<br>40x/1.2 W corr. Apo<br>63x/1.4 Oil Plan-Apo<br>63x/1.2 W Apo | ||
+ | |illumination=Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>594 nm HeNe laser<br>633 nm HeNe laser | ||
}} | }} |
Latest revision as of 15:43, 3 February 2016
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
microscope | Zeiss - Axiovert 200M, Inverted stand, inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
objectives | |||
illumination | |||
detection | point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
reflectors | EX 323-380, EM 417-469 (Zeiss FS00wf) EX 422-443, EM 458-498 (Zeiss FS38wf) EX 451-489, EM 496-546 (Zeiss FS47wf) EX 531-581, EM LP617 (Zeiss FS49) | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan | ||
software | - | ||
incubation | Stage incubator (T) + objective heater | ||
links |
| ||
inv.nr. | 404687 SIP:71680 10.1.15.75 10.1.39.223"404687 SIP:71680 10.1.15.75 10.1.39.223" cannot be used as a page name in this wiki. |