CZ3 - Zeiss LSM 405/594
From BioDIP
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| description = Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs and transmitted light detector. | | description = Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs and transmitted light detector. | ||
| applications = dual color imaging of EGFP + mCherry and routine confocal imaging of suitable dye combinations | | applications = dual color imaging of EGFP + mCherry and routine confocal imaging of suitable dye combinations | ||
− | | microscope = inverted stand, | + | | microscope = inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC |
| objectives = 10x/0.45 Plan-Neo<br>25x/0.8 Imm corr. Plan-Neo<br>40x/1.3 Oil Plan-Neo<br>40x/1.2 W corr. Apo<br>63x/1.4 Oil Plan-Apo<br>63x/1.2 W Apo | | objectives = 10x/0.45 Plan-Neo<br>25x/0.8 Imm corr. Plan-Neo<br>40x/1.3 Oil Plan-Neo<br>40x/1.2 W corr. Apo<br>63x/1.4 Oil Plan-Apo<br>63x/1.2 W Apo | ||
| illumination = Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>594 nm HeNe laser<br>633 nm HeNe laser | | illumination = Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>594 nm HeNe laser<br>633 nm HeNe laser | ||
| detection = point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | | detection = point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
| reflectors = EX 323-380, EM 417-469 (Zeiss FS00wf)<br>EX 422-443, EM 458-498 (Zeiss FS38wf)<br>EX 451-489, EM 496-546 (Zeiss FS47wf)<br>EX 531-581, EM LP617 (Zeiss FS49) | | reflectors = EX 323-380, EM 417-469 (Zeiss FS00wf)<br>EX 422-443, EM 458-498 (Zeiss FS38wf)<br>EX 451-489, EM 496-546 (Zeiss FS47wf)<br>EX 531-581, EM LP617 (Zeiss FS49) | ||
− | | features = simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation | + | | features = simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan |
| incubation = mobile stage incubator | | incubation = mobile stage incubator | ||
| link1 = [http://spreadsheets.google.com/pub?key=pFACvabR4mSlISzehf0x6JQ laser power] | | link1 = [http://spreadsheets.google.com/pub?key=pFACvabR4mSlISzehf0x6JQ laser power] |
Revision as of 11:53, 4 June 2009
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | , inverted stand, inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
objectives |
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illumination |
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detection | point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
reflectors | EX 323-380, EM 417-469 (Zeiss FS00wf) EX 422-443, EM 458-498 (Zeiss FS38wf) EX 451-489, EM 496-546 (Zeiss FS47wf) EX 531-581, EM LP617 (Zeiss FS49) | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan | ||
software | |||
incubation | mobile stage incubator | ||
links |
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inv.nr. | 404687 SIP:71680"404687 SIP:71680" cannot be used as a page name in this wiki. |
[[Category:{{{type}}}]]