CZ4 - Zeiss LSM DuoScan

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{{Equipment
 
{{Equipment
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| system-number       = 11
+
|system-number=MPI-CZ4
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| system-name         = Zeiss LSM DuoScan
+
|system-name=Zeiss LSM DuoScan
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| category             = Laser Scanning Confocal
+
|type=Not listed
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| stand                = inverted
+
|category=Laser Scanning Confocal
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| image                = MPI11.jpg
+
|facility=MPI-CBG LMF
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| facility             = MPI-CBG LMF
+
|building=MPI-CBG
−
| facility_image      = logo-lmf.jpg
+
|room=038A
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| building            = MPI-CBG
+
|Facility-logo=MPI-CBG-Lmf-logo-big.png
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| room                = 038A
+
|Institute-logo=Logo MPI-CBG.jpg
−
| description         = Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition.
+
|description=Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition. AIM SYSTEM 2435000381
−
| applications         = spectral imaging, routine dye combinations, [[tile scan]], multi position scan
+
|applications=spectral imaging, routine dye combinations, [[tile scan]], multi position scan
−
| microscope           = motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
+
|image=CZ4 - Zeiss LSM DuoScan.jpg
−
| objectives          = 10x/0.3<br>20x/0.8 Apo<br>40x/1.2 W Apo<br>63x/1.4 Oil Apo<br>100x/1.45 Oil<br>60x/1.2 W Apo
+
|stand-name=Zeiss - Axio Observer
−
| illumination        = Halogen<br>Metal-Halide<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>561 nm DPSS laser<br>633 nm HeNe laser
+
|stand=Inverted
−
| detection           = point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector
+
|microscope=motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
−
| reflectors           = 1. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488049-0000-000  Filter Set 49]. G365 * FT395 *  BP445/50 <br>2. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488013-0000-000  Filter Set 13]. BP 470/20 * FT493 * BP505-530 <br>3. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488015-0000-000 Filter Set 15]. BP546/12 * FT580 * LP590.
+
|obj1=Zeiss Plan-Apochromat 10x 0.45
−
| features             = simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
+
|obj2=Zeiss Plan-Apochromat 20x 0.8
−
| incubation           = mobile stage incubator
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|obj3=Zeiss C-Apochromat 40x 1.2 W
−
| link1               = [http://spreadsheets.google.com/pub?key=pFACvabR4mSl8mKAtkf_cZg LPM]
+
|obj4=Zeiss Plan-Apochromat 63x 1.4 Oil
−
| link2               = [https://spreadsheets.google.com/pub?key=pMZJXCgd5HNtGjGuWC1GTCQ scan-head configuration]
+
|obj5=Zeiss LCI-PLAN Neofluar 25x0.8 Oil/Glyc/Water DIC 0-0.17
−
| inv                 = -
+
|obj6=Zeiss EC Plan-Neofluar 40x 1.3 Oil
 +
|ill1=Transmitted Light (Halogen)
 +
|ill2=Fluorescence (Metal Halide, X-Cite, 120W)
 +
|ill3=Laser Diode 405 nm
 +
|ill4=Laser Argon Multiline 458, 477, 488, 514 nm
 +
|ill5=Laser DPSS 561 nm
 +
|ill6=Laser HeNe 633 nm
 +
|ill7=Laser DPSS 488 nm
 +
|detection=point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector
 +
|reflectors=1. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488049-0000-000  Filter Set 49]. G365 * FT395 *  BP445/50 <br>2. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488013-0000-000  Filter Set 13]. BP 470/20 * FT493 * BP505-530 <br>3. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488015-0000-000 Filter Set 15]. BP546/12 * FT580 * LP590.
 +
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
 +
|software=-
 +
|incubation=Stage incubator (T) + objective heater
 +
|link1=[http://spreadsheets.google.com/pub?key=pFACvabR4mSl8mKAtkf_cZg LPM]
 +
|link2=[https://spreadsheets.google.com/pub?key=pMZJXCgd5HNtGjGuWC1GTCQ scan-head configuration]
 +
|inv=AIM SYSTEM 2435000381    10.1.145.12
 +
|facility_image=logo-lmf.jpg
 +
|objectives=10x/0.3<br>20x/0.8 Apo<br>40x/1.2 W Apo<br>63x/1.4 Oil Apo<br>100x/1.45 Oil<br>60x/1.2 W Apo
 +
|illumination=Halogen<br>Metal-Halide<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>561 nm DPSS laser<br>633 nm HeNe laser
 
}}
 
}}

Latest revision as of 15:44, 3 February 2016

Suitable for [[applications::spectral imaging, routine dye combinations, tile scan, multi position scan]].
CZ4 - Zeiss LSM DuoScan.jpg


[edit] Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 038A)

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope Zeiss - Axio Observer, Inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss C-Apochromat 40x 1.2 W
Zeiss Plan-Apochromat 63x 1.4 Oil
Zeiss LCI-PLAN Neofluar 25x0.8 Oil/Glyc/Water DIC 0-0.17
Zeiss EC Plan-Neofluar 40x 1.3 Oil
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide, X-Cite, 120W)
Laser Diode 405 nm
Laser Argon Multiline 458, 477, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
Laser DPSS 488 nm
detection point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector
reflectors [[reflectors::1. Filter Set 49. G365 * FT395 * BP445/50
2. Filter Set 13. BP 470/20 * FT493 * BP505-530
3. Filter Set 15. BP546/12 * FT580 * LP590.]]
features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
software -
incubation Stage incubator (T) + objective heater
links
  • [ manual]
  • [LPM laser power values]
inv.nr. AIM SYSTEM 2435000381 10.1.145.12


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