CZ4 - Zeiss LSM DuoScan
From BioDIP
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{{Equipment | {{Equipment | ||
| − | | system-number | + | |system-number=MPI11 |
| − | | system-name | + | |system-name=Zeiss LSM DuoScan |
| − | | category | + | |type=Microscope |
| − | + | |category=Laser Scanning Confocal | |
| − | + | |facility=MPI-CBG LMF | |
| − | | facility | + | |building=MPI-CBG |
| − | + | |room=038A | |
| − | | building | + | |facility_image=logo-lmf.jpg |
| − | | room | + | |description=Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition. |
| − | | description | + | |applications=spectral imaging, routine dye combinations, [[tile scan]], multi position scan |
| − | | applications | + | |image=MPI11.jpg |
| − | | microscope | + | |stand=Inverted |
| − | | objectives | + | |microscope=motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC |
| − | | illumination | + | |objectives=10x/0.3<br>20x/0.8 Apo<br>40x/1.2 W Apo<br>63x/1.4 Oil Apo<br>100x/1.45 Oil<br>60x/1.2 W Apo |
| − | | detection | + | |illumination=Halogen<br>Metal-Halide<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>561 nm DPSS laser<br>633 nm HeNe laser |
| − | | reflectors | + | |detection=point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector |
| − | | features | + | |reflectors=1. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488049-0000-000 Filter Set 49]. G365 * FT395 * BP445/50 <br>2. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488013-0000-000 Filter Set 13]. BP 470/20 * FT493 * BP505-530 <br>3. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488015-0000-000 Filter Set 15]. BP546/12 * FT580 * LP590. |
| − | | incubation | + | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan |
| − | | link1 | + | |incubation=stage incubator (T) + objective heater |
| − | | link2 | + | |link1=[http://spreadsheets.google.com/pub?key=pFACvabR4mSl8mKAtkf_cZg LPM] |
| − | | inv | + | |link2=[https://spreadsheets.google.com/pub?key=pMZJXCgd5HNtGjGuWC1GTCQ scan-head configuration] |
| + | |inv=- | ||
| + | |obj1=Zeiss Plan-Apochromat 10x 0.45 | ||
| + | |obj2=Zeiss Plan-Apochromat 20x 0.8 | ||
| + | |obj3=Zeiss C-Apochromat 40x 1.2 W | ||
| + | |obj4=Zeiss Plan-Apochromat 63x 1.4 Oil | ||
| + | |obj5=Zeiss alpha Plan-Fluar 100x 1.45 Oil | ||
| + | |obj6=Zeiss C-Apochromat 63x 1.2 W | ||
| + | |obj7=- | ||
| + | |obj8=- | ||
}} | }} | ||
Revision as of 17:15, 1 September 2009
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | , Inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
| objectives | |||
| illumination |
| ||
| detection | point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
| reflectors | [[reflectors::1. Filter Set 49. G365 * FT395 * BP445/50 2. Filter Set 13. BP 470/20 * FT493 * BP505-530 3. Filter Set 15. BP546/12 * FT580 * LP590.]] | ||
| features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan | ||
| software | |||
| incubation | stage incubator (T) + objective heater | ||
| links |
| ||
| inv.nr. | - |
