CZ4 - Zeiss LSM DuoScan

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|building=MPI-CBG
 
|building=MPI-CBG
 
|room=038A
 
|room=038A
|facility_image=logo-lmf.jpg
 
 
|description=Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition. AIM SYSTEM 2435000381
 
|description=Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition. AIM SYSTEM 2435000381
 
|applications=spectral imaging, routine dye combinations, [[tile scan]], multi position scan
 
|applications=spectral imaging, routine dye combinations, [[tile scan]], multi position scan
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|link1=[http://spreadsheets.google.com/pub?key=pFACvabR4mSl8mKAtkf_cZg LPM]
 
|link1=[http://spreadsheets.google.com/pub?key=pFACvabR4mSl8mKAtkf_cZg LPM]
 
|link2=[https://spreadsheets.google.com/pub?key=pMZJXCgd5HNtGjGuWC1GTCQ scan-head configuration]
 
|link2=[https://spreadsheets.google.com/pub?key=pMZJXCgd5HNtGjGuWC1GTCQ scan-head configuration]
|inv=AIM SYSTEM 2435000381
+
|inv=AIM SYSTEM 2435000381   10.1.145.12
 +
|facility_image=logo-lmf.jpg
 
|objectives=10x/0.3<br>20x/0.8 Apo<br>40x/1.2 W Apo<br>63x/1.4 Oil Apo<br>100x/1.45 Oil<br>60x/1.2 W Apo
 
|objectives=10x/0.3<br>20x/0.8 Apo<br>40x/1.2 W Apo<br>63x/1.4 Oil Apo<br>100x/1.45 Oil<br>60x/1.2 W Apo
 
|illumination=Halogen<br>Metal-Halide<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>561 nm DPSS laser<br>633 nm HeNe laser
 
|illumination=Halogen<br>Metal-Halide<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>561 nm DPSS laser<br>633 nm HeNe laser
 
}}
 
}}

Revision as of 20:37, 1 September 2011

Suitable for [[applications::spectral imaging, routine dye combinations, tile scan, multi position scan]].
MPI11.jpg


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 038A)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axio Observer, Inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss C-Apochromat 40x 1.2 W
Zeiss Plan-Apochromat 63x 1.4 Oil
Zeiss alpha Plan-Fluar 100x 1.45 Oil
Zeiss EC Plan-Neofluar 40x 1.3 Oil
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide, X-Cite, 120W)
Laser Diode 405 nm
Laser Argon Multiline 458, 477, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
Laser DPSS 488 nm
detection point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector
reflectors [[reflectors::1. Filter Set 49. G365 * FT395 * BP445/50
2. Filter Set 13. BP 470/20 * FT493 * BP505-530
3. Filter Set 15. BP546/12 * FT580 * LP590.]]
features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
software -
incubation Stage incubator (T) + objective heater
links
  • [ manual]
  • [LPM laser power values]
inv.nr. AIM SYSTEM 2435000381 10.1.145.12



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