CZ4 - Zeiss LSM DuoScan
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{{Equipment | {{Equipment | ||
− | |system-number= | + | |system-number=MPI-CZ4 |
|system-name=Zeiss LSM DuoScan | |system-name=Zeiss LSM DuoScan | ||
− | |type= | + | |type=Not listed |
|category=Laser Scanning Confocal | |category=Laser Scanning Confocal | ||
|facility=MPI-CBG LMF | |facility=MPI-CBG LMF | ||
|building=MPI-CBG | |building=MPI-CBG | ||
|room=038A | |room=038A | ||
− | | | + | |Facility-logo=MPI-CBG-Lmf-logo-big.png |
− | |description=Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition. | + | |Institute-logo=Logo MPI-CBG.jpg |
+ | |description=Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition. AIM SYSTEM 2435000381 | ||
|applications=spectral imaging, routine dye combinations, [[tile scan]], multi position scan | |applications=spectral imaging, routine dye combinations, [[tile scan]], multi position scan | ||
− | |image= | + | |image=CZ4 - Zeiss LSM DuoScan.jpg |
+ | |stand-name=Zeiss - Axio Observer | ||
|stand=Inverted | |stand=Inverted | ||
|microscope=motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | |microscope=motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
− | | | + | |obj1=Zeiss Plan-Apochromat 10x 0.45 |
− | | | + | |obj2=Zeiss Plan-Apochromat 20x 0.8 |
+ | |obj3=Zeiss C-Apochromat 40x 1.2 W | ||
+ | |obj4=Zeiss Plan-Apochromat 63x 1.4 Oil | ||
+ | |obj5=Zeiss LCI-PLAN Neofluar 25x0.8 Oil/Glyc/Water DIC 0-0.17 | ||
+ | |obj6=Zeiss EC Plan-Neofluar 40x 1.3 Oil | ||
+ | |ill1=Transmitted Light (Halogen) | ||
+ | |ill2=Fluorescence (Metal Halide, X-Cite, 120W) | ||
+ | |ill3=Laser Diode 405 nm | ||
+ | |ill4=Laser Argon Multiline 458, 477, 488, 514 nm | ||
+ | |ill5=Laser DPSS 561 nm | ||
+ | |ill6=Laser HeNe 633 nm | ||
+ | |ill7=Laser DPSS 488 nm | ||
|detection=point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | |detection=point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
|reflectors=1. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488049-0000-000 Filter Set 49]. G365 * FT395 * BP445/50 <br>2. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488013-0000-000 Filter Set 13]. BP 470/20 * FT493 * BP505-530 <br>3. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488015-0000-000 Filter Set 15]. BP546/12 * FT580 * LP590. | |reflectors=1. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488049-0000-000 Filter Set 49]. G365 * FT395 * BP445/50 <br>2. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488013-0000-000 Filter Set 13]. BP 470/20 * FT493 * BP505-530 <br>3. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488015-0000-000 Filter Set 15]. BP546/12 * FT580 * LP590. | ||
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan | ||
− | |incubation= | + | |software=- |
+ | |incubation=Stage incubator (T) + objective heater | ||
|link1=[http://spreadsheets.google.com/pub?key=pFACvabR4mSl8mKAtkf_cZg LPM] | |link1=[http://spreadsheets.google.com/pub?key=pFACvabR4mSl8mKAtkf_cZg LPM] | ||
|link2=[https://spreadsheets.google.com/pub?key=pMZJXCgd5HNtGjGuWC1GTCQ scan-head configuration] | |link2=[https://spreadsheets.google.com/pub?key=pMZJXCgd5HNtGjGuWC1GTCQ scan-head configuration] | ||
− | |inv= | + | |inv=AIM SYSTEM 2435000381 10.1.145.12 |
− | | | + | |facility_image=logo-lmf.jpg |
− | | | + | |objectives=10x/0.3<br>20x/0.8 Apo<br>40x/1.2 W Apo<br>63x/1.4 Oil Apo<br>100x/1.45 Oil<br>60x/1.2 W Apo |
− | + | |illumination=Halogen<br>Metal-Halide<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>561 nm DPSS laser<br>633 nm HeNe laser | |
− | + | ||
− | + | ||
− | + | ||
− | | | + | |
− | + | ||
}} | }} |
Latest revision as of 15:44, 3 February 2016
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
microscope | Zeiss - Axio Observer, Inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
objectives | |||
illumination | |||
detection | point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
reflectors | [[reflectors::1. Filter Set 49. G365 * FT395 * BP445/50 2. Filter Set 13. BP 470/20 * FT493 * BP505-530 3. Filter Set 15. BP546/12 * FT580 * LP590.]] | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan | ||
software | - | ||
incubation | Stage incubator (T) + objective heater | ||
links |
| ||
inv.nr. | AIM SYSTEM 2435000381 10.1.145.12 |