CZ5 - Zeiss LSM 700 inverted

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Line 25: Line 25:
 
|ill6=Fluorescence (Metal Halide)
 
|ill6=Fluorescence (Metal Halide)
 
|detection=2 PMTs<br>
 
|detection=2 PMTs<br>
1 transmission detector
+
No transmission detector
 
|reflectors=Dapi : EX 350/50 ; BS 395 ; EM LP 420 <br>
 
|reflectors=Dapi : EX 350/50 ; BS 395 ; EM LP 420 <br>
 
FITC : EX 480/30 ; BS 505 ; EM 535/40 <br>
 
FITC : EX 480/30 ; BS 505 ; EM 535/40 <br>

Revision as of 12:30, 17 July 2012

It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with."Routine confocal workhorse for simple, standard and tile scan jobs. It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with." cannot be used as a page name in this wiki.

Suitable for 2D images, z-stacks, time lapse, bleaching and tile scans.
250px


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 038a)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axio Observer, Inverted stand, -
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Neofluar 25x 0.8 Imm Corr
Zeiss LCI Plan-Neofluar 63x 1.3 Imm Corr
illumination
Laser Diode 405 nm
Laser DPSS 488 nm
Laser DPSS 555nm
Laser DPSS 639nm
Transmitted Light (Halogen)
Fluorescence (Metal Halide)
detection 2 PMTs

No transmission detector

reflectors Dapi : EX 350/50 ; BS 395 ; EM LP 420

FITC : EX 480/30 ; BS 505 ; EM 535/40
DsRed : EX 545/10 ; BS 555 ; EM 600/70
GFP/RFP : EX  ; BS  ; EM

features -
software ZEN 2010
incubation Not available
links
  • manual
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0 10.1.145.33



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