Revision as of 18:32, 3 February 2016 (view source) BrittaSD (Talk | contribs) (creation of page, copy whole page content from CZ7)		Revision as of 18:40, 3 February 2016 (view source) BrittaSD (Talk | contribs) (changed all relevant information to that of CZ6 (as far as I know)) Newer edit →
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	|Facility-logo=MPI-CBG-Lmf-logo-big.png		|Facility-logo=MPI-CBG-Lmf-logo-big.png
	|Institute-logo=Logo MPI-CBG.jpg		|Institute-logo=Logo MPI-CBG.jpg
−	|description=Zeiss LSM 880 upright	+	|description=Zeiss LSM 880 inverted
	single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2PMTs) and transmitted light detector.		single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2PMTs) and transmitted light detector.
	Airy scan detector for high resolution imaging.		Airy scan detector for high resolution imaging.
	Motorized stage for multi-point acquisition		Motorized stage for multi-point acquisition
	|applications=Optical sectioning for 3D reconstruction; fast bleaching samples; high-resolution imaging; multi-color imaging; linear and spectral unmixing of overlapping emission spectra; tile scan; multi position scan; FRAP; FRET		|applications=Optical sectioning for 3D reconstruction; fast bleaching samples; high-resolution imaging; multi-color imaging; linear and spectral unmixing of overlapping emission spectra; tile scan; multi position scan; FRAP; FRET
−	|image=CZ7 - Zeiss LSM 880 Airy upright.jpg	+	|image=CZ6 - Zeiss LSM 880 Airy inverted.jpg
−	|stand-name=Zeiss - Axio Imager	+	|stand-name=Zeiss - Axio Observer.Z1
−	|stand=Upright	+	|stand=Inverted
−	|microscope=motorized XY stage, motorized Z-drive	+	|microscope=motorized XY stage, motorized Z-drive, Piezo Z stage
	|obj1=Zeiss Plan-Apochromat 10x 0.45		|obj1=Zeiss Plan-Apochromat 10x 0.45
	|obj2=Zeiss Plan-Apochromat 20x 0.8		|obj2=Zeiss Plan-Apochromat 20x 0.8
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	|ill1=Fluorescence (Metal Halide, HXP, 120W)		|ill1=Fluorescence (Metal Halide, HXP, 120W)
	|ill2=Transmitted Light (Halogen)		|ill2=Transmitted Light (Halogen)
−	|ill3=Laser Diode 405 nm	+	|ill3=Laser DPSS 355 nm
−	|ill4=Laser Argon Multiline 458, 477, 488, 514 nm	+	|ill4=Laser Diode 405 nm
−	|ill5=Laser DPSS 561 nm	+	|ill5=Laser Argon Multiline 458, 477, 488, 496, 514 nm
−	|ill6=Laser HeNe 594 nm	+	|ill6=Laser DPSS 561 nm
−	|ill7=Laser HeNe 633 nm	+	|ill7=Laser HeNe 594 nm
		+	|ill8=Laser HeNe 633 nm
	|detection=32 GaAsP PMT channels <br>		|detection=32 GaAsP PMT channels <br>
	2 PMTs<br>		2 PMTs<br>
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	|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan, Airy scan detection		|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan, Airy scan detection
	|software=Zen 2 black		|software=Zen 2 black
−	|incubation=Not available	+	|incubation=Cage incubator (T) + Stage incubator (T+CO2)
−	|link1=https://docs.google.com/spreadsheets/d/1Ez1kZJetwazShfgL_269vb-v4a8Xf8HGT-0zW551clg/edit?usp=sharing	+	|link1=https://docs.google.com/spreadsheets/d/1gV_odyApUK5cyqumOLOC4yOJrALN_55VRJBrvWzHPNU/edit?usp=sharing
	}}		}}

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Revision as of 18:40, 3 February 2016

single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2PMTs) and transmitted light detector. Airy scan detector for high resolution imaging. Motorized stage for multi-point acquisition"Zeiss LSM 880 inverted single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2PMTs) and transmitted light detector. Airy scan detector for high resolution imaging. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.

Suitable for Optical sectioning for 3D reconstruction; fast bleaching samples; high-resolution imaging; multi-color imaging; linear and spectral unmixing of overlapping emission spectra; tile scan; multi position scan; FRAP; FRET.

Directions

MPI-CBG LMF (MPI-CBG, 038A)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Zeiss - Axio Observer.Z1, Inverted stand, motorized XY stage, motorized Z-drive, Piezo Z stage
objectives	Zeiss Plan-Apochromat 10x 0.45 Zeiss Plan-Apochromat 20x 0.8 Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC Zeiss W Plan-Apochromat 40x 1.0 Zeiss Plan-Apochromat 63x 1.4 Oil DIC Zeiss i LCI Plan-Neofluar 63x 1.3 Imm Korr DIC
illumination	Fluorescence (Metal Halide, HXP, 120W) Transmitted Light (Halogen) Laser DPSS 355 nm Laser Diode 405 nm Laser Argon Multiline 458, 477, 488, 496, 514 nm Laser DPSS 561 nm Laser HeNe 594 nm Laser HeNe 633 nm
detection	32 GaAsP PMT channels 2 PMTs 1 32-channel Airy Scan detector transmission detector
reflectors	Zeiss set 49: DAPI Zeiss set 38 Endow: GFP Zeiss set 45: HQ Texas Red
features	simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan, Airy scan detection
software	Zen 2 black
incubation	Cage incubator (T) + Stage incubator (T+CO2)
links	[ manual] laser power values [ beam path] [ advanced system check]
inv.nr.

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