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| Line 11: |
Line 11: |
| | |description=Zeiss LSM 880 upright | | |description=Zeiss LSM 880 upright |
| | Single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2PMTs) and transmitted light detector. | | Single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2PMTs) and transmitted light detector. |
| | + | Airy scan detector for high resolution imaging. |
| | Motorized stage for multi-point acquisition | | Motorized stage for multi-point acquisition |
| − | |applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS | + | |applications=Optical sectioning for 3D reconstruction; fast bleaching samples; high-resolution imaging; multi-color imaging; linear and spectral unmixing of overlapping emission spectra; tile scan; multi position scan; FRAP; FRET. |
| | |image=MZ1 - Zeiss 2photon upright.jpg | | |image=MZ1 - Zeiss 2photon upright.jpg |
| | |stand-name=Zeiss - Axio Imager | | |stand-name=Zeiss - Axio Imager |
| microscope
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Zeiss - Axio Imager, Upright stand, motorized XY stage, motorized Z-drive,
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| objectives
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- Zeiss EC Plan-Neofluar 10x 0.3
- Zeiss C-Apochromat 40x 1.2 W
- Zeiss Plan-Apochromat 40x 1.0 W DIC
- Zeiss N-Achroplan 20x 0.5 W
- Zeiss Plan-Apochromat 20x 0.8
- Zeiss Plan-Apochromat 10x 0.45
- Zeiss Plan-Apochromat 63x 1.4 Oil DIC
- Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC
- Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC
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| illumination
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- Fluorescence (Metal Halide, PRIOR, 200W)
- Transmitted Light (Halogen)
- Laser Diode 405 nm
- Laser Argon Multiline 458, 477, 488, 514 nm
- Laser DPSS 561 nm
- Laser HeNe 594 nm
- Laser HeNe 633 nm
- Laser Multiphoton Chameleon 700-1064 nm
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| detection
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Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting
2 photon detection: Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP and RFP
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| reflectors
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-
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| features
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simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
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| software
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Zen 2010 B
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| incubation
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Not available
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| links
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- [ beam path]
- [ advanced system check]
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| inv.nr.
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AIM System 2501000445 10.1.145.41 (IPv4)
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