Revision as of 13:26, 3 February 2016 (view source) BrittaSD (Talk | contribs) ← Older edit		Latest revision as of 12:49, 15 September 2016 (view source) Nitzsche (Talk | contribs)
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	{{Equipment		{{Equipment
−	|system-number=MPI-MZ1	+	|system-number=MPI- CZ7
−	|system-name=Zeiss 2-photon upright	+	|system-name=Zeiss LSM 880 Airy upright
	|type=Microscope		|type=Microscope
	|category=Laser Scanning Confocal		|category=Laser Scanning Confocal
Line 10:		Line 10:
	|Institute-logo=Logo MPI-CBG.jpg		|Institute-logo=Logo MPI-CBG.jpg
	|description=Zeiss LSM 880 upright		|description=Zeiss LSM 880 upright
−	Single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2PMTs) and transmitted light detector.	+	single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2PMTs) and transmitted light detector.
	Airy scan detector for high resolution imaging.		Airy scan detector for high resolution imaging.
	Motorized stage for multi-point acquisition		Motorized stage for multi-point acquisition
	|applications=Optical sectioning for 3D reconstruction; fast bleaching samples; high-resolution imaging; multi-color imaging; linear and spectral unmixing of overlapping emission spectra; tile scan; multi position scan; FRAP; FRET		|applications=Optical sectioning for 3D reconstruction; fast bleaching samples; high-resolution imaging; multi-color imaging; linear and spectral unmixing of overlapping emission spectra; tile scan; multi position scan; FRAP; FRET
−	|image=MZ1 - Zeiss 2photon upright.jpg	+	|image=CZ7 - Zeiss LSM 880 Airy upright.jpg
	|stand-name=Zeiss - Axio Imager		|stand-name=Zeiss - Axio Imager
	|stand=Upright		|stand=Upright
−	|microscope=motorized XY stage, motorized Z-drive,	+	|microscope=motorized XY stage, motorized Z-drive
−	|obj1=Zeiss EC Plan-Neofluar 10x 0.3	+	|obj1=Zeiss Plan-Apochromat 10x 0.45
−	|obj2=Zeiss C-Apochromat 40x 1.2 W	+	|obj2=Zeiss Plan-Apochromat 20x 0.8
−	|obj3=Zeiss Plan-Apochromat 40x 1.0 W DIC	+	|obj3=Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC
−	|obj4=Zeiss N-Achroplan 20x 0.5 W	+	|obj4=Zeiss W Plan-Apochromat 40x 1.0
−	|obj5=Zeiss Plan-Apochromat 20x 0.8	+	|obj5=Zeiss Plan-Apochromat 63x 1.4 Oil DIC
−	|obj6=Zeiss Plan-Apochromat 10x 0.45	+	|obj6=Zeiss i LCI Plan-Neofluar 63x 1.3 Imm Korr DIC
−	|obj7=Zeiss Plan-Apochromat 63x 1.4 Oil DIC	+	|ill1=Fluorescence (Metal Halide, HXP, 120W)
−	|obj8=Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC	+
−	|obj9=Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC	+
−	|ill1=Fluorescence (Metal Halide, PRIOR, 200W)	+
	|ill2=Transmitted Light (Halogen)		|ill2=Transmitted Light (Halogen)
	|ill3=Laser Diode 405 nm		|ill3=Laser Diode 405 nm
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	|ill6=Laser HeNe 594 nm		|ill6=Laser HeNe 594 nm
	|ill7=Laser HeNe 633 nm		|ill7=Laser HeNe 633 nm
−	|ill8=Laser Multiphoton Chameleon 700-1064 nm	+	|detection=32 GaAsP PMT channels <br>
−	|detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br>	+	2 PMTs<br>
−	'''2 photon detection:''' Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP and RFP	+	1 32-channel Airy Scan detector <br>
−	|reflectors=-	+	transmission detector
−	|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan	+	|reflectors=Zeiss set 49: DAPI<br>
−	|software=Zen 2010 B	+	Zeiss set 38 Endow: GFP<br>
		+	Zeiss set 45: HQ Texas Red
		+	|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan, Airy scan detection
		+	|software=Zen 2 black
	|incubation=Not available		|incubation=Not available
−	|link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-MZ1_-_Zeiss_2photon_upright_-_startup_%26_shutdown	+	|link1=https://docs.google.com/spreadsheets/d/1Ez1kZJetwazShfgL_269vb-v4a8Xf8HGT-0zW551clg/pubhtml?gid=0&single=true
−	|link1=https://docs.google.com/spreadsheet/pub?hl=en_US&hl=en_US&key=0AiAvGnTuH4LedC1CZjIxa05nTnFOM2Ixb0RvdGltaEE&output=html	+
−	|inv=AIM System 2501000445  10.1.145.41 (IPv4)	+
	}}		}}

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Latest revision as of 12:49, 15 September 2016

single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2PMTs) and transmitted light detector. Airy scan detector for high resolution imaging. Motorized stage for multi-point acquisition"Zeiss LSM 880 upright single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2PMTs) and transmitted light detector. Airy scan detector for high resolution imaging. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.

Suitable for Optical sectioning for 3D reconstruction; fast bleaching samples; high-resolution imaging; multi-color imaging; linear and spectral unmixing of overlapping emission spectra; tile scan; multi position scan; FRAP; FRET.

[edit] Directions

MPI-CBG LMF (MPI-CBG, 038A)

[edit] Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

[edit] Details

microscope	Zeiss - Axio Imager, Upright stand, motorized XY stage, motorized Z-drive
objectives	Zeiss Plan-Apochromat 10x 0.45 Zeiss Plan-Apochromat 20x 0.8 Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC Zeiss W Plan-Apochromat 40x 1.0 Zeiss Plan-Apochromat 63x 1.4 Oil DIC Zeiss i LCI Plan-Neofluar 63x 1.3 Imm Korr DIC
illumination	Fluorescence (Metal Halide, HXP, 120W) Transmitted Light (Halogen) Laser Diode 405 nm Laser Argon Multiline 458, 477, 488, 514 nm Laser DPSS 561 nm Laser HeNe 594 nm Laser HeNe 633 nm
detection	32 GaAsP PMT channels 2 PMTs 1 32-channel Airy Scan detector transmission detector
reflectors	Zeiss set 49: DAPI Zeiss set 38 Endow: GFP Zeiss set 45: HQ Texas Red
features	simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan, Airy scan detection
software	Zen 2 black
incubation	Not available
links	[ manual] laser power values [ beam path] [ advanced system check]
inv.nr.

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