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| |system-name=Zeiss 2-photon upright | | |system-name=Zeiss 2-photon upright |
| |type=Microscope | | |type=Microscope |
− | |category=Multiphoton Laser Scanning | + | |category=Laser Scanning Confocal |
| |facility=MPI-CBG LMF | | |facility=MPI-CBG LMF |
| |building=MPI-CBG | | |building=MPI-CBG |
− | |room=038C | + | |room=038A |
| |Facility-logo=MPI-CBG-Lmf-logo-big.png | | |Facility-logo=MPI-CBG-Lmf-logo-big.png |
| |Institute-logo=Logo MPI-CBG.jpg | | |Institute-logo=Logo MPI-CBG.jpg |
− | |description=Zeiss LSM 780 NLO | + | |description=Zeiss LSM 880 upright |
− | *Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
| + | *Single photon point scanning confocal system with Quasar detector (one 32 spectral detection channels GaAsP detector plus 2PMTs) and transmitted light detector. |
− | *Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition | + | Motorized stage for multi-point acquisition |
| |applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS | | |applications=imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS |
| |image=MZ1 - Zeiss 2photon upright.jpg | | |image=MZ1 - Zeiss 2photon upright.jpg |
microscope
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Zeiss - Axio Imager, Upright stand, motorized XY stage, motorized Z-drive,
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objectives
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- Zeiss EC Plan-Neofluar 10x 0.3
- Zeiss C-Apochromat 40x 1.2 W
- Zeiss Plan-Apochromat 40x 1.0 W DIC
- Zeiss N-Achroplan 20x 0.5 W
- Zeiss Plan-Apochromat 20x 0.8
- Zeiss Plan-Apochromat 10x 0.45
- Zeiss Plan-Apochromat 63x 1.4 Oil DIC
- Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC
- Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC
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illumination
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- Fluorescence (Metal Halide, PRIOR, 200W)
- Transmitted Light (Halogen)
- Laser Diode 405 nm
- Laser Argon Multiline 458, 477, 488, 514 nm
- Laser DPSS 561 nm
- Laser HeNe 594 nm
- Laser HeNe 633 nm
- Laser Multiphoton Chameleon 700-1064 nm
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-
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detection
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Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting
2 photon detection: Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP and RFP
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reflectors
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-
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features
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simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
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software
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Zen 2010 B
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incubation
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Not available
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links
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- [ beam path]
- [ advanced system check]
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inv.nr.
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AIM System 2501000445 10.1.145.41 (IPv4)
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