Checklist for introducing new users to the MTZ Imaging facility
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* Stay with user until he/she feels save to use the system independently. | * Stay with user until he/she feels save to use the system independently. | ||
* Invite user to contact the MTZ-Imaging about questions / problems / changes in their imaging projects at any time. | * Invite user to contact the MTZ-Imaging about questions / problems / changes in their imaging projects at any time. | ||
| − | * Administative doings (MTZ-Imaging [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging | + | * Administative doings (MTZ-Imaging [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging website], Scheduling Database, Mailing List, access to the room, payment and billing) |
Revision as of 13:33, 16 February 2010
Contents |
First Contact
- send email to lichtmikroskopie.mtz(at)tu-dresden.de or contact the MTZ-Imaging by Phone +49 351 458 16426
- give some informations about your project and sample
1) What is the sample? (Organism? Tissue? Cell Type? Living? Fixed?)
2) How is it prepared? (Grown on glass? Tissue section? Living ?)
3) How is it mounted? Under a (proper exactly 0.17 mm) coverglass? Underwater in an agarose well? special chamber?
4) What Staining / Dyes / contrast agents will you use? (Fluorescence and/or transmitted light?)
5) What special informations do you want to get out of your sample (Do you need 2D or 3D images (z stacks)?; Do you need time series? If so, what time resolution / frames per seconds do you need? What is the size of the field of view that you need?; How big are the objects you are interested in?)
- MTZ-Imagigng will arrange a personal meeting for your first introduction and recommend which system fits well for your experiments Leica SP 5 or Zeiss 510
Introductions
First Introduction
Without your own sample
Estimated time: usually around 2hours
- Introduction to hardware: how to switch on/off.
- Introduction to software: how to properly acquire an image, z-stack, time series (it might sometimes not be possible to cover all of that in the 1st intro).
- Data handling: explain about original file formats and metadata; advantages/disadvantages of storing data on either hard drive or fileserver
- Data safety: Point out that the MTZ-Imaging holds no responsibility for data whatsoever!
Important: in order to keep the disk free for imaging - data older than 2 month can be deleted on an irregular basis without warning.
- Safety regulations: laser safety, S1 safety, correct use of wastebins.
- How to clean objectives.
Second Introduction
With own sample!!!
Estimated time: project dependent, usually around 3 hours to give some time for working alone on the system
- Recap contents of first intro
- Introduce user to things that couldn't be covered in the first session
- Help user to set up the system for their own imaging experiment.
- Stay with user until he/she feels save to use the system independently.
- Invite user to contact the MTZ-Imaging about questions / problems / changes in their imaging projects at any time.
- Administative doings (MTZ-Imaging website, Scheduling Database, Mailing List, access to the room, payment and billing)
