Checklist for introducing new users to the MTZ Imaging facility
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| − | [[MTZ02 - Leica TCS SP5]] | + | == First Contact == |
| + | |||
| + | * Send email to lichtmikroskopie.mtz(at)tu-dresden.de or contact the MTZ-Imaging by Phone +49 351 458 16426 | ||
| + | * Give some information about your project and sample | ||
| + | ** What is the sample? (Organism? Tissue? Cell Type? Living? Fixed?) | ||
| + | ** How is it prepared? (Grown on glass? Tissue section? Living ?) | ||
| + | ** How is it mounted? Under a (proper exactly 0.17 mm) coverglass? Underwater in an agarose well? special chamber? | ||
| + | ** What Staining / Dyes / contrast agents will you use? (Fluorescence and/or transmitted light?) | ||
| + | ** What special information do you want to get out of your sample (Do you need 2D or 3D images (z stacks)?; Do you need time series? If so, what time resolution / frames per seconds do you need? What is the size of the field of view that you need?; How big are the objects you are interested in?) | ||
| + | |||
| + | * MTZ-Imagigng will arrange a personal meeting for your first introduction and recommend which system fits well for your experiments: [[MTZ02 - Leica TCS SP5|Leica SP 5]] or [[MTZ01 - Zeiss LSM 510|Zeiss 510]] | ||
| + | |||
| + | |||
| + | == Introductions == | ||
| + | |||
| + | === First Introduction === | ||
| + | |||
| + | '' Without your own sample '' | ||
| + | |||
| + | '' Estimated time: usually around 2hours '' | ||
| + | |||
| + | * Introduction to hardware: how to switch on/off. | ||
| + | * Introduction to software: how to properly acquire an image, z-stack, time series (it might sometimes not be possible to cover all of that in the 1st intro). | ||
| + | * Data handling: explain about original file formats and metadata; advantages/disadvantages of storing data on either hard drive or fileserver | ||
| + | * Data safety: Point out that the MTZ-Imaging holds no responsibility for data whatsoever! | ||
| + | '''Important:''' in order to keep the disk free for imaging - data older than 2 month can be deleted on an irregular basis without warning. | ||
| + | * Safety regulations: laser safety, S1 safety, correct use of wastebins. | ||
| + | * How to clean objectives. | ||
| + | |||
| + | |||
| + | === Second Introduction === | ||
| + | |||
| + | '' With own sample!!! '' | ||
| + | |||
| + | '' Estimated time: project dependent, usually around 3 hours to give some time for working alone on the system '' | ||
| + | |||
| + | * Recap contents of first intro | ||
| + | * Introduce user to things that couldn't be covered in the first session | ||
| + | * Help user to set up the system for their own imaging experiment. | ||
| + | * Stay with user until he/she feels save to use the system independently. | ||
| + | * Invite user to contact the MTZ-Imaging about questions / problems / changes in their imaging projects at any time. | ||
| + | * Administrative isues (MTZ-Imaging [http://tu-dresden.de/die_tu_dresden/fakultaeten/medizinische_fakultaet/mtz/mtzImaging website], Scheduling Database, Mailing List, access to the room, payment and billing) | ||
Latest revision as of 15:53, 16 February 2010
Contents |
[edit] First Contact
- Send email to lichtmikroskopie.mtz(at)tu-dresden.de or contact the MTZ-Imaging by Phone +49 351 458 16426
- Give some information about your project and sample
- What is the sample? (Organism? Tissue? Cell Type? Living? Fixed?)
- How is it prepared? (Grown on glass? Tissue section? Living ?)
- How is it mounted? Under a (proper exactly 0.17 mm) coverglass? Underwater in an agarose well? special chamber?
- What Staining / Dyes / contrast agents will you use? (Fluorescence and/or transmitted light?)
- What special information do you want to get out of your sample (Do you need 2D or 3D images (z stacks)?; Do you need time series? If so, what time resolution / frames per seconds do you need? What is the size of the field of view that you need?; How big are the objects you are interested in?)
- MTZ-Imagigng will arrange a personal meeting for your first introduction and recommend which system fits well for your experiments: Leica SP 5 or Zeiss 510
[edit] Introductions
[edit] First Introduction
Without your own sample
Estimated time: usually around 2hours
- Introduction to hardware: how to switch on/off.
- Introduction to software: how to properly acquire an image, z-stack, time series (it might sometimes not be possible to cover all of that in the 1st intro).
- Data handling: explain about original file formats and metadata; advantages/disadvantages of storing data on either hard drive or fileserver
- Data safety: Point out that the MTZ-Imaging holds no responsibility for data whatsoever!
Important: in order to keep the disk free for imaging - data older than 2 month can be deleted on an irregular basis without warning.
- Safety regulations: laser safety, S1 safety, correct use of wastebins.
- How to clean objectives.
[edit] Second Introduction
With own sample!!!
Estimated time: project dependent, usually around 3 hours to give some time for working alone on the system
- Recap contents of first intro
- Introduce user to things that couldn't be covered in the first session
- Help user to set up the system for their own imaging experiment.
- Stay with user until he/she feels save to use the system independently.
- Invite user to contact the MTZ-Imaging about questions / problems / changes in their imaging projects at any time.
- Administrative isues (MTZ-Imaging website, Scheduling Database, Mailing List, access to the room, payment and billing)
