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== Effect of the CLSM pinhole == | == Effect of the CLSM pinhole == | ||
− | + | * The same region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image). | |
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[[Image:Pinholeseries_9.jpg|100px]] | [[Image:Pinholeseries_9.jpg|100px]] | ||
+ | [[Image:Pinholeseries_8.jpg|100px]] | ||
+ | [[Image:Pinholeseries_7.jpg|100px]] | ||
+ | [[Image:Pinholeseries_6.jpg|100px]] | ||
+ | [[Image:Pinholeseries_5.jpg|100px]] | ||
+ | [[Image:Pinholeseries_4.jpg|100px]] | ||
+ | [[Image:Pinholeseries_3.jpg|100px]] | ||
+ | [[Image:Pinholeseries_2.jpg|100px]] | ||
+ | [[Image:Pinholeseries_1.jpg|100px]] | ||
+ | * Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-580nm detection, 0.2µm Z-stepsize | ||
== Cleaning an objective makes sense == | == Cleaning an objective makes sense == |
Revision as of 14:22, 23 April 2009
Effect of the CLSM pinhole
- The same region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image).
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-580nm detection, 0.2µm Z-stepsize
Cleaning an objective makes sense
- First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.
- Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm