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[[Image:Pinholeseries_1.jpg|100px]] | [[Image:Pinholeseries_1.jpg|100px]] | ||
| − | * Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-580nm detection, 0.2µm Z-stepsize | + | * Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-580nm detection, 0.2µm Z-stepsize, Bitplane Imaris |
== Cleaning an objective makes sense == | == Cleaning an objective makes sense == | ||
Revision as of 14:24, 23 April 2009
Effect of the CLSM pinhole
- The same region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image).
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-580nm detection, 0.2µm Z-stepsize, Bitplane Imaris
Cleaning an objective makes sense
- First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.
- Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm
