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== Effect of the CLSM pinhole == | == Effect of the CLSM pinhole == | ||
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+ | === XY === | ||
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+ | * High resolution image, acquired with decreasing pinhole diameter from 8 airy units to 0.6 airy units. | ||
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+ | [[Image:63xinvitrogen-4-PH8.jpg|100px]] | ||
+ | [[Image:63xinvitrogen-4-PH7.jpg|100px]] | ||
+ | [[Image:63xinvitrogen-4-PH6.jpg|100px]] | ||
+ | [[Image:63xinvitrogen-4-PH5.jpg|100px]] | ||
+ | [[Image:63xinvitrogen-4-PH4.jpg|100px]] | ||
+ | [[Image:63xinvitrogen-4-PH3.jpg|100px]] | ||
+ | [[Image:63xinvitrogen-4-PH2.jpg|100px]] | ||
+ | [[Image:63xinvitrogen-4-PH1.jpg|100px]] | ||
+ | [[Image:63xinvitrogen-4-PH0.8.jpg|100px]] | ||
+ | [[Image:63xinvitrogen-4-PH0.6.jpg|100px]] | ||
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+ | * Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594nm excitation, LP610nm detection | ||
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+ | === XYZ === | ||
* The same region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image). | * The same region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image). |
Revision as of 14:36, 23 April 2009
Contents |
Effect of the CLSM pinhole
XY
- High resolution image, acquired with decreasing pinhole diameter from 8 airy units to 0.6 airy units.
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594nm excitation, LP610nm detection
XYZ
- The same region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image).
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-580nm detection, 0.2µm Z-stepsize, Bitplane Imaris
Cleaning an objective makes sense
- First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.
- Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm