Gallery
From BioDIP
(Difference between revisions)
m |
|||
Line 20: | Line 20: | ||
=== XYZ === | === XYZ === | ||
− | * | + | * One region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image). |
[[Image:Pinholeseries_9.jpg|100px]] | [[Image:Pinholeseries_9.jpg|100px]] |
Revision as of 14:37, 23 April 2009
Contents |
Effect of the CLSM pinhole
XY
- High resolution image, acquired with decreasing pinhole diameter from 8 airy units to 0.6 airy units.
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594nm excitation, LP610nm detection
XYZ
- One region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image).
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-580nm detection, 0.2µm Z-stepsize, Bitplane Imaris
Cleaning an objective makes sense
- First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.
- Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm