Gallery
From BioDIP
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=== overview images === | === overview images === | ||
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| + | * Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 airy units to 1 airy unit. | ||
[[Image:Confocaldualcolor_8.jpg|100px]] | [[Image:Confocaldualcolor_8.jpg|100px]] | ||
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[[Image:Confocaldualcolor_2.jpg|100px]] | [[Image:Confocaldualcolor_2.jpg|100px]] | ||
[[Image:Confocaldualcolor_1.jpg|100px]] | [[Image:Confocaldualcolor_1.jpg|100px]] | ||
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| + | * Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection | ||
=== XY === | === XY === | ||
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[[Image:63xinvitrogen-4-PH0.6.jpg|100px]] | [[Image:63xinvitrogen-4-PH0.6.jpg|100px]] | ||
| − | * Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, | + | * Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection |
=== XYZ === | === XYZ === | ||
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[[Image:Pinholeseries_1.jpg|100px]] | [[Image:Pinholeseries_1.jpg|100px]] | ||
| − | * Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, | + | * Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris |
== Cleaning an objective makes sense == | == Cleaning an objective makes sense == | ||
Revision as of 14:57, 23 April 2009
Contents |
Effect of the CLSM pinhole
overview images
- Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 airy units to 1 airy unit.
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection
XY
- High resolution image, acquired with decreasing pinhole diameter from 8 airy units to 0.6 airy units.
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection
XYZ
- One region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image).
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris
Cleaning an objective makes sense
- First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.
- Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm
