Gallery
From BioDIP
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* One region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image). | * One region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image). | ||
+ | * Available as [http://vimeo.com/4900159 Video] | ||
[[Image:Pinholeseries_9.jpg|100px]] | [[Image:Pinholeseries_9.jpg|100px]] |
Revision as of 15:43, 29 May 2009
Contents |
Effect of the CLSM pinhole
overview images
- Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 airy units to 1 airy unit.
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection
XY
- High resolution image, acquired with decreasing pinhole diameter from 8 airy units to 0.6 airy units.
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection
XYZ
- One region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image).
- Available as Video
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris
Cleaning an objective makes sense
- First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.
- Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm