Gallery
From BioDIP
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* Available as [http://vimeo.com/4900784 Video] | * Available as [http://vimeo.com/4900784 Video] | ||
− | + | <gallery perrow=8> | |
− | + | Image:Confocaldualcolor_8.jpg|8 Airy Units | |
− | + | Image:Confocaldualcolor_7.jpg|7 Airy Units | |
− | + | Image:Confocaldualcolor_6.jpg|6 Airy Units | |
− | + | Image:Confocaldualcolor_5.jpg|5 Airy Units | |
− | + | Image:Confocaldualcolor_4.jpg|4 Airy Units | |
− | + | Image:Confocaldualcolor_3.jpg|3 Airy Units | |
− | + | Image:Confocaldualcolor_2.jpg|2 Airy Units | |
+ | Image:Confocaldualcolor_1.jpg|1 Airy Unit | ||
+ | </gallery> | ||
* Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection | * Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection |
Revision as of 13:30, 3 July 2009
Contents |
Effect of the CLSM pinhole
overview images
- Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 airy units to 1 airy unit.
- Available as Video
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection
XY
- High resolution image, acquired with decreasing pinhole diameter from 8 airy units to 0.6 airy units.
- Available as Video
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection
XYZ
- One region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image).
- Available as Video
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris
Cleaning an objective makes sense
- First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.
- Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm