Gallery

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m (2nd+3rd+4th gallery style)
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* Available as [http://vimeo.com/4901422 Video]
 
* Available as [http://vimeo.com/4901422 Video]
  
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[[Image:63xinvitrogen-4-PH8.jpg|100px]]
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<gallery perrow=5>
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[[Image:63xinvitrogen-4-PH7.jpg|100px]]
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Image:63xinvitrogen-4-PH8.jpg|8 Airy Units
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[[Image:63xinvitrogen-4-PH6.jpg|100px]]
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Image:63xinvitrogen-4-PH7.jpg|7 Airy Units
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[[Image:63xinvitrogen-4-PH5.jpg|100px]]
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Image:63xinvitrogen-4-PH6.jpg|6 Airy Units
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[[Image:63xinvitrogen-4-PH4.jpg|100px]]
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Image:63xinvitrogen-4-PH5.jpg|5 Airy Units
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[[Image:63xinvitrogen-4-PH3.jpg|100px]]
+
Image:63xinvitrogen-4-PH4.jpg|4 Airy Units
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[[Image:63xinvitrogen-4-PH2.jpg|100px]]
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Image:63xinvitrogen-4-PH3.jpg|3 Airy Units
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[[Image:63xinvitrogen-4-PH1.jpg|100px]]
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Image:63xinvitrogen-4-PH2.jpg|2 Airy Units
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[[Image:63xinvitrogen-4-PH0.8.jpg|100px]]
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Image:63xinvitrogen-4-PH1.jpg|1 Airy Unit
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[[Image:63xinvitrogen-4-PH0.6.jpg|100px]]
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Image:63xinvitrogen-4-PH0.8.jpg|0.8 Airy Unit
 +
Image:63xinvitrogen-4-PH0.6.jpg|0.6 Airy Unit
 +
</gallery>
  
 
* Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection
 
* Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection
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=== XYZ ===
 
=== XYZ ===
  
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* One region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image).
+
* One region of the sample was imaged with decreasing pinhole diameter, starting from 8 airy units (first image) down to 1 airy units (last image).
 
* Available as [http://vimeo.com/4900159 Video]
 
* Available as [http://vimeo.com/4900159 Video]
  
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[[Image:Pinholeseries_9.jpg|100px]]
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<gallery perrow=4>
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[[Image:Pinholeseries_8.jpg|100px]]
+
Image:Pinholeseries_8.jpg|8 Airy Units
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[[Image:Pinholeseries_7.jpg|100px]]
+
Image:Pinholeseries_7.jpg|7 Airy Units
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[[Image:Pinholeseries_6.jpg|100px]]
+
Image:Pinholeseries_6.jpg|6 Airy Units
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[[Image:Pinholeseries_5.jpg|100px]]
+
Image:Pinholeseries_5.jpg|5 Airy Units
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[[Image:Pinholeseries_4.jpg|100px]]
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Image:Pinholeseries_4.jpg|4 Airy Units
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[[Image:Pinholeseries_3.jpg|100px]]
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Image:Pinholeseries_3.jpg|3 Airy Units
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[[Image:Pinholeseries_2.jpg|100px]]
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Image:Pinholeseries_2.jpg|2 Airy Units
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[[Image:Pinholeseries_1.jpg|100px]]
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Image:Pinholeseries_1.jpg|1 Airy Unit
 +
</gallery>
  
 
* Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris
 
* Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris
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* First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.
 
* First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.
  
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[[Image:2009-04-23_63xdirty-scale.jpg|256px|dirty]] [[Image:2009-04-23_63xclean-scale.jpg|256px|clean]]
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<gallery perrow=2>
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Image:2009-04-23_63xdirty-scale.jpg|w/o lens cleaning
 +
Image:2009-04-23_63xclean-scale.jpg|w/ simple lens cleaning (2 wipes with Whatman(r) paper and 70% EtOH)
 +
</gallery>
  
 
* Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm
 
* Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm

Revision as of 13:36, 3 July 2009

Contents

Effect of the CLSM pinhole

overview images

  • Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 airy units to 1 airy unit.
  • Available as Video

  • 8 Airy Units

  • 7 Airy Units

  • 6 Airy Units

  • 5 Airy Units

  • 4 Airy Units

  • 3 Airy Units

  • 2 Airy Units

  • 1 Airy Unit

  • Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection

XY

  • High resolution image, acquired with decreasing pinhole diameter from 8 airy units to 0.6 airy units.
  • Available as Video

  • 8 Airy Units

  • 7 Airy Units

  • 6 Airy Units

  • 5 Airy Units

  • 4 Airy Units

  • 3 Airy Units

  • 2 Airy Units

  • 1 Airy Unit

  • 0.8 Airy Unit

  • 0.6 Airy Unit

  • Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection

XYZ

  • One region of the sample was imaged with decreasing pinhole diameter, starting from 8 airy units (first image) down to 1 airy units (last image).
  • Available as Video

  • 8 Airy Units

  • 7 Airy Units

  • 6 Airy Units

  • 5 Airy Units

  • 4 Airy Units

  • 3 Airy Units

  • 2 Airy Units

  • 1 Airy Unit

  • Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris

Cleaning an objective makes sense

  • First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.

  • w/o lens cleaning

  • w/ simple lens cleaning (2 wipes with Whatman(r) paper and 70% EtOH)

  • Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm
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