Gallery
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* Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 airy units to 1 airy unit. | * Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 airy units to 1 airy unit. | ||
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* High resolution image, acquired with decreasing pinhole diameter from 8 airy units to 0.6 airy units. | * High resolution image, acquired with decreasing pinhole diameter from 8 airy units to 0.6 airy units. | ||
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* One region of the sample was imaged with decreasing pinhole diameter, starting from 8 airy units (first image) down to 1 airy units (last image). | * One region of the sample was imaged with decreasing pinhole diameter, starting from 8 airy units (first image) down to 1 airy units (last image). | ||
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<gallery perrow=4> | <gallery perrow=4> | ||
Revision as of 13:36, 23 September 2009
Contents |
Images acquired with our equipment
Effect of the CLSM pinhole
overview images
- Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 airy units to 1 airy unit.
8 Airy Units
7 Airy Units
6 Airy Units
5 Airy Units
4 Airy Units
3 Airy Units
2 Airy Units
1 Airy Unit
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection
XY
- High resolution image, acquired with decreasing pinhole diameter from 8 airy units to 0.6 airy units.
8 Airy Units
7 Airy Units
6 Airy Units
5 Airy Units
4 Airy Units
3 Airy Units
2 Airy Units
1 Airy Unit
0.8 Airy Unit
0.6 Airy Unit
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection
XYZ
- One region of the sample was imaged with decreasing pinhole diameter, starting from 8 airy units (first image) down to 1 airy units (last image).
8 Airy Units
7 Airy Units
6 Airy Units
5 Airy Units
4 Airy Units
3 Airy Units
2 Airy Units
1 Airy Unit
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris
Cleaning an objective makes sense
w/o lens cleaning
w/ simple lens cleaning (2 wipes with Whatman(r) paper and 70% EtOH)
- Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm
Photos
Peter Evennett
"Principles of Light Microscopy" course 2007
course announcement
Peter Evennett and Humberto at the optical bench
room overview - 2nd floor galeria
Peter at his demo microscope
it's all about lenses
Jan
the optical bench
Peter at the condenser
demonstration of refractive index with a cup, a coin and water
Peter Evennett
Peter & Peter, setting up the demo microscope
Tim Cross, finally at the pipette
PhD Course
2005
sampling
up to 4 years, simplified
cribs
final test
