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+ | == Images acquired with our equipment == | ||
+ | |||
+ | === Effect of the CLSM pinhole === | ||
+ | |||
+ | ==== overview images ==== | ||
+ | |||
+ | * Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 [[Airy unit]]s to 1 [[Airy unit]]. | ||
+ | |||
+ | <gallery perrow=4> | ||
+ | Image:Confocaldualcolor_8.jpg|8 [[Airy unit]]s | ||
+ | Image:Confocaldualcolor_7.jpg|7 [[Airy unit]]s | ||
+ | Image:Confocaldualcolor_6.jpg|6 [[Airy unit]]s | ||
+ | Image:Confocaldualcolor_5.jpg|5 [[Airy unit]]s | ||
+ | Image:Confocaldualcolor_4.jpg|4 [[Airy unit]]s | ||
+ | Image:Confocaldualcolor_3.jpg|3 [[Airy unit]]s | ||
+ | Image:Confocaldualcolor_2.jpg|2 [[Airy unit]]s | ||
+ | Image:Confocaldualcolor_1.jpg|1 [[Airy unit]] | ||
+ | </gallery> | ||
+ | |||
+ | {{#widget:Vimeo|id=4900784}} | ||
+ | |||
+ | * Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection | ||
+ | |||
+ | ==== XY ==== | ||
+ | |||
+ | * High resolution image, acquired with decreasing pinhole diameter from 8 [[Airy unit]]s to 0.6 [[Airy unit]]s. | ||
+ | |||
<gallery> | <gallery> | ||
− | + | Image:63xinvitrogen-4-PH8.jpg|8 [[Airy unit]]s | |
− | + | Image:63xinvitrogen-4-PH7.jpg|7 [[Airy unit]]s | |
+ | Image:63xinvitrogen-4-PH6.jpg|6 [[Airy unit]]s | ||
+ | Image:63xinvitrogen-4-PH5.jpg|5 [[Airy unit]]s | ||
+ | Image:63xinvitrogen-4-PH4.jpg|4 [[Airy unit]]s | ||
+ | Image:63xinvitrogen-4-PH3.jpg|3 [[Airy unit]]s | ||
+ | Image:63xinvitrogen-4-PH2.jpg|2 [[Airy unit]]s | ||
+ | Image:63xinvitrogen-4-PH1.jpg|1 [[Airy unit]] | ||
+ | Image:63xinvitrogen-4-PH0.8.jpg|0.8 [[Airy unit]]s | ||
+ | Image:63xinvitrogen-4-PH0.6.jpg|0.6 [[Airy unit]]s | ||
+ | </gallery> | ||
+ | |||
+ | {{#widget:Vimeo|id=4901422}} | ||
+ | |||
+ | * Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection | ||
+ | |||
+ | ==== XYZ ==== | ||
+ | |||
+ | * One region of the sample was imaged with decreasing pinhole diameter, starting from 8 airy units (first image) down to 1 airy units (last image). | ||
+ | |||
+ | <gallery perrow=4> | ||
+ | Image:Pinholeseries_8.jpg|8 [[Airy unit]]s | ||
+ | Image:Pinholeseries_7.jpg|7 [[Airy unit]]s | ||
+ | Image:Pinholeseries_6.jpg|6 [[Airy unit]]s | ||
+ | Image:Pinholeseries_5.jpg|5 [[Airy unit]]s | ||
+ | Image:Pinholeseries_4.jpg|4 [[Airy unit]]s | ||
+ | Image:Pinholeseries_3.jpg|3 [[Airy unit]]s | ||
+ | Image:Pinholeseries_2.jpg|2 [[Airy unit]]s | ||
+ | Image:Pinholeseries_1.jpg|1 [[Airy unit]] | ||
+ | </gallery> | ||
+ | |||
+ | {{#widget:Vimeo|id=4900159}} | ||
+ | |||
+ | * Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris | ||
+ | |||
+ | === Cleaning an objective makes sense === | ||
+ | |||
+ | <gallery perrow=2> | ||
+ | Image:2009-04-23_63xdirty-scale.jpg|w/o lens cleaning | ||
+ | Image:2009-04-23_63xclean-scale.jpg|w/ simple lens cleaning (2 wipes with Whatman(r) paper and 70% EtOH) | ||
+ | </gallery> | ||
+ | |||
+ | * Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm | ||
+ | |||
+ | == Photos == | ||
+ | === Peter Evennett === | ||
+ | ==== "Principles of Light Microscopy" course 2007 ==== | ||
+ | <gallery perrow=4> | ||
+ | Image:Course-poster.jpg | course announcement | ||
+ | Image:DSC0312.jpg | Peter Evennett and Humberto at the optical bench | ||
+ | Image:DSC0320.jpg | room overview - 2nd floor galeria | ||
+ | Image:DSC0324.jpg | Peter at his demo microscope | ||
+ | Image:DSC0327.jpg | it's all about lenses | ||
+ | Image:DSC0328.jpg | Jan | ||
+ | Image:DSC0333.jpg | the optical bench | ||
+ | Image:DSC0335.jpg | Peter at the condenser | ||
+ | Image:DSC0341.jpg | demonstration of refractive index with a cup, a coin and water | ||
+ | Image:DSC0343.jpg | Peter Evennett | ||
+ | Image:DSC0344.jpg | Peter & Peter, setting up the demo microscope | ||
+ | Image:DSC0347.jpg | Tim Cross, finally at the pipette | ||
+ | </gallery> | ||
+ | * [[Peter_Evennett_-_Abbe_Diffraction_Experiments|video by Peter Evennett: Abbe's diffraction experiments]] | ||
+ | === PhD Course === | ||
+ | ==== 2005 ==== | ||
+ | <gallery perrow=4> | ||
+ | Image:DSCN3198.JPG | sampling | ||
+ | Image:DSCN3203.JPG | up to 4 years, simplified | ||
+ | Image:DSCN3205.JPG | cribs | ||
+ | Image:DSCN3208.JPG | final test | ||
</gallery> | </gallery> |
Latest revision as of 22:38, 13 March 2011
Contents |
[edit] Images acquired with our equipment
[edit] Effect of the CLSM pinhole
[edit] overview images
- Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 Airy units to 1 Airy unit.
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection
[edit] XY
- High resolution image, acquired with decreasing pinhole diameter from 8 Airy units to 0.6 Airy units.
0.8 Airy units
0.6 Airy units
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection
[edit] XYZ
- One region of the sample was imaged with decreasing pinhole diameter, starting from 8 airy units (first image) down to 1 airy units (last image).
- Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris
[edit] Cleaning an objective makes sense
- Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm