Gallery

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== Effect of the CLSM pinhole ==
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== Images acquired with our equipment ==
  
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=== overview images ===
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=== Effect of the CLSM pinhole ===
  
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* Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 airy units to 1 airy unit.
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==== overview images ====
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* Available as [http://vimeo.com/4900784 Video]
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 +
* Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 [[Airy unit]]s to 1 [[Airy unit]].
  
 
<gallery perrow=4>
 
<gallery perrow=4>
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Image:Confocaldualcolor_8.jpg|8 Airy Units
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Image:Confocaldualcolor_8.jpg|8 [[Airy unit]]s
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Image:Confocaldualcolor_7.jpg|7 Airy Units
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Image:Confocaldualcolor_7.jpg|7 [[Airy unit]]s
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Image:Confocaldualcolor_6.jpg|6 Airy Units
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Image:Confocaldualcolor_6.jpg|6 [[Airy unit]]s
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Image:Confocaldualcolor_5.jpg|5 Airy Units
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Image:Confocaldualcolor_5.jpg|5 [[Airy unit]]s
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Image:Confocaldualcolor_4.jpg|4 Airy Units
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Image:Confocaldualcolor_4.jpg|4 [[Airy unit]]s
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Image:Confocaldualcolor_3.jpg|3 Airy Units
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Image:Confocaldualcolor_3.jpg|3 [[Airy unit]]s
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Image:Confocaldualcolor_2.jpg|2 Airy Units
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Image:Confocaldualcolor_2.jpg|2 [[Airy unit]]s
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Image:Confocaldualcolor_1.jpg|1 Airy Unit
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Image:Confocaldualcolor_1.jpg|1 [[Airy unit]]
 
</gallery>
 
</gallery>
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{{#widget:Vimeo|id=4900784}}
  
 
* Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection
 
* Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection
  
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=== XY ===
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==== XY ====
  
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* High resolution image, acquired with decreasing pinhole diameter from 8 airy units to 0.6 airy units.
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* High resolution image, acquired with decreasing pinhole diameter from 8 [[Airy unit]]s to 0.6 [[Airy unit]]s.
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* Available as [http://vimeo.com/4901422 Video]
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[[Image:63xinvitrogen-4-PH8.jpg|100px]]
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<gallery>
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[[Image:63xinvitrogen-4-PH7.jpg|100px]]
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Image:63xinvitrogen-4-PH8.jpg|8 [[Airy unit]]s
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[[Image:63xinvitrogen-4-PH6.jpg|100px]]
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Image:63xinvitrogen-4-PH7.jpg|7 [[Airy unit]]s
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[[Image:63xinvitrogen-4-PH5.jpg|100px]]
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Image:63xinvitrogen-4-PH6.jpg|6 [[Airy unit]]s
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[[Image:63xinvitrogen-4-PH4.jpg|100px]]
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Image:63xinvitrogen-4-PH5.jpg|5 [[Airy unit]]s
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[[Image:63xinvitrogen-4-PH3.jpg|100px]]
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Image:63xinvitrogen-4-PH4.jpg|4 [[Airy unit]]s
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[[Image:63xinvitrogen-4-PH2.jpg|100px]]
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Image:63xinvitrogen-4-PH3.jpg|3 [[Airy unit]]s
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[[Image:63xinvitrogen-4-PH1.jpg|100px]]
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Image:63xinvitrogen-4-PH2.jpg|2 [[Airy unit]]s
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[[Image:63xinvitrogen-4-PH0.8.jpg|100px]]
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Image:63xinvitrogen-4-PH1.jpg|1 [[Airy unit]]
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[[Image:63xinvitrogen-4-PH0.6.jpg|100px]]
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Image:63xinvitrogen-4-PH0.8.jpg|0.8 [[Airy unit]]s
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Image:63xinvitrogen-4-PH0.6.jpg|0.6 [[Airy unit]]s
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</gallery>
 +
 
 +
{{#widget:Vimeo|id=4901422}}
  
 
* Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection
 
* Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection
  
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=== XYZ ===
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==== XYZ ====
  
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* One region of the sample was imaged with decreasing pinhole diameter, starting from 9 airy units (first image) down to 1 airy units (last image).
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* One region of the sample was imaged with decreasing pinhole diameter, starting from 8 airy units (first image) down to 1 airy units (last image).
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* Available as [http://vimeo.com/4900159 Video]
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[[Image:Pinholeseries_9.jpg|100px]]
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<gallery perrow=4>
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[[Image:Pinholeseries_8.jpg|100px]]
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Image:Pinholeseries_8.jpg|8 [[Airy unit]]s
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[[Image:Pinholeseries_7.jpg|100px]]
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Image:Pinholeseries_7.jpg|7 [[Airy unit]]s
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[[Image:Pinholeseries_6.jpg|100px]]
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Image:Pinholeseries_6.jpg|6 [[Airy unit]]s
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[[Image:Pinholeseries_5.jpg|100px]]
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Image:Pinholeseries_5.jpg|5 [[Airy unit]]s
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[[Image:Pinholeseries_4.jpg|100px]]
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Image:Pinholeseries_4.jpg|4 [[Airy unit]]s
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[[Image:Pinholeseries_3.jpg|100px]]
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Image:Pinholeseries_3.jpg|3 [[Airy unit]]s
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[[Image:Pinholeseries_2.jpg|100px]]
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Image:Pinholeseries_2.jpg|2 [[Airy unit]]s
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[[Image:Pinholeseries_1.jpg|100px]]
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Image:Pinholeseries_1.jpg|1 [[Airy unit]]
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</gallery>
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 +
{{#widget:Vimeo|id=4900159}}
  
 
* Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris
 
* Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris
  
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== Cleaning an objective makes sense ==
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=== Cleaning an objective makes sense ===
  
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* First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.
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<gallery perrow=2>
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Image:2009-04-23_63xdirty-scale.jpg|w/o lens cleaning
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[[Image:2009-04-23_63xdirty-scale.jpg|256px|dirty]] [[Image:2009-04-23_63xclean-scale.jpg|256px|clean]]
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Image:2009-04-23_63xclean-scale.jpg|w/ simple lens cleaning (2 wipes with Whatman(r) paper and 70% EtOH)
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</gallery>
  
 
* Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm
 
* Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm
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== Photos ==
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=== Peter Evennett ===
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==== "Principles of Light Microscopy" course 2007 ====
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<gallery perrow=4>
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Image:Course-poster.jpg | course announcement
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Image:DSC0312.jpg | Peter Evennett and Humberto at the optical bench
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Image:DSC0320.jpg | room overview - 2nd floor galeria
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Image:DSC0324.jpg | Peter at his demo microscope
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Image:DSC0327.jpg | it's all about lenses
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Image:DSC0328.jpg | Jan
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Image:DSC0333.jpg | the optical bench
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Image:DSC0335.jpg | Peter at the condenser
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Image:DSC0341.jpg | demonstration of refractive index with a cup, a coin and water
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Image:DSC0343.jpg | Peter Evennett
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Image:DSC0344.jpg | Peter & Peter, setting up the demo microscope
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Image:DSC0347.jpg | Tim Cross, finally at the pipette
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</gallery>
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* [[Peter_Evennett_-_Abbe_Diffraction_Experiments|video by Peter Evennett: Abbe's diffraction experiments]]
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=== PhD Course ===
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==== 2005 ====
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<gallery perrow=4>
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Image:DSCN3198.JPG | sampling
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Image:DSCN3203.JPG | up to 4 years, simplified
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Image:DSCN3205.JPG | cribs
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Image:DSCN3208.JPG | final test
 +
</gallery>

Latest revision as of 22:38, 13 March 2011

Contents

[edit] Images acquired with our equipment

[edit] Effect of the CLSM pinhole

[edit] overview images

  • Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 Airy units to 1 Airy unit.

  • Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection

[edit] XY

  • High resolution image, acquired with decreasing pinhole diameter from 8 Airy units to 0.6 Airy units.

  • Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection

[edit] XYZ

  • One region of the sample was imaged with decreasing pinhole diameter, starting from 8 airy units (first image) down to 1 airy units (last image).

  • Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris

[edit] Cleaning an objective makes sense

  • w/o lens cleaning

  • w/ simple lens cleaning (2 wipes with Whatman(r) paper and 70% EtOH)

  • Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm

[edit] Photos

[edit] Peter Evennett

[edit] "Principles of Light Microscopy" course 2007

  • course announcement

  • Peter Evennett and Humberto at the optical bench

  • room overview - 2nd floor galeria

  • Peter at his demo microscope

  • it's all about lenses

  • Jan

  • the optical bench

  • Peter at the condenser

  • demonstration of refractive index with a cup, a coin and water

  • Peter Evennett

  • Peter & Peter, setting up the demo microscope

  • Tim Cross, finally at the pipette

[edit] PhD Course

[edit] 2005

  • sampling

  • up to 4 years, simplified

  • cribs

  • final test

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