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Cleaning an objective makes sense

  • First image taken w/o cleaning the lens. Second image after two wipes with Whatman(r) paper and cleaning agent.

dirty clean

  • Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm
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